Detailed information of TA system TA044428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 5311245..5311908
• Replicon: chromosome
• Accession: NZ_AP028047
• Organism: Phytobacter diazotrophicus strain TA9759

Toxin (Protein)

• Gene name: tad
• Uniprot ID: A0A348DL83
• Locus tag: QUE80_RS25535
• Protein ID: WP_039077908.1
• Coordinates: 5311245..5311589 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: A0A348DL84
• Locus tag: QUE80_RS25540
• Protein ID: WP_071194355.1
• Coordinates: 5311591..5311908 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE80_RS25515 (5306922)	5306922..5308328	-	1407	WP_039077905.1	replicative DNA helicase	-
QUE80_RS25520 (5308387)	5308387..5309370	+	984	WP_039077906.1	quinone oxidoreductase	-
QUE80_RS25525 (5309659)	5309659..5309901	-	243	WP_039077907.1	envelope stress response protein PspG	-
QUE80_RS25530 (5310054)	5310054..5311052	-	999	WP_039077935.1	tRNA dihydrouridine(20/20a) synthase DusA	-
QUE80_RS25535 (5311245)	5311245..5311589	+	345	WP_039077908.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE80_RS25540 (5311591)	5311591..5311908	+	318	WP_071194355.1	helix-turn-helix transcriptional regulator	Antitoxin
QUE80_RS25545 (5311950)	5311950..5313266	-	1317	WP_039077910.1	conjugal transfer protein TraF	-
QUE80_RS25550 (5313539)	5313539..5314054	+	516	WP_039077912.1	zinc uptake transcriptional repressor Zur	-
QUE80_RS25555 (5314162)	5314162..5314371	-	210	WP_039077936.1	CsbD family protein	-
QUE80_RS25560 (5314469)	5314469..5314657	+	189	WP_071194358.1	hypothetical protein	-
QUE80_RS25565 (5314632)	5314632..5315246	-	615	WP_039077914.1	transcriptional repressor LexA	-
QUE80_RS25570 (5315355)	5315355..5315723	-	369	WP_079497650.1	diacylglycerol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12655.46 Da
• Isoelectric Point: 9.5820

>T44428 WP_039077908.1 NZ_AP028047:5311245-5311589 [Phytobacter diazotrophicus]
MKPLYWVGSSKKDLQSLPEDVQDVFGYALHLAQDGGKHPQAKPLKGFGGAGVLEVVEDYCTDTYRAVYTLKFRQAVYVLH
AFQKKSSSGMQTPKSDIEKIRERLKAAEAHARGA

Nucleotide

• Length: 345 bp

>T44428 NZ_AP028047:5311245-5311589 [Phytobacter diazotrophicus]
ATGAAGCCACTGTATTGGGTGGGGAGCAGCAAAAAAGATCTTCAGTCTCTGCCGGAGGATGTTCAGGATGTTTTTGGTTA
TGCGTTGCATCTCGCACAAGACGGAGGTAAGCATCCCCAGGCAAAACCGTTAAAAGGATTTGGGGGCGCAGGTGTGCTGG
AGGTGGTCGAAGACTATTGCACCGATACTTATCGCGCGGTTTATACCCTGAAATTCAGACAGGCGGTCTATGTGCTACAT
GCATTTCAGAAGAAGTCGTCATCCGGTATGCAAACCCCCAAATCTGATATAGAAAAAATTCGAGAACGTCTTAAGGCAGC
AGAAGCCCATGCCAGAGGAGCATAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11644.64 Da
• Isoelectric Point: 11.1929

>AT44428 WP_071194355.1 NZ_AP028047:5311591-5311908 [Phytobacter diazotrophicus]
MSQNIDISSGNVYADIGLDNADEMQVKAQLATTIGNIIKSRRLTQVEAAKLLGMTQPRLSNMLRGQFRGISEVKMLECLT
RLGRDVQIVVGRPRRTPGRMKVVFA

Nucleotide

• Length: 318 bp

>AT44428 NZ_AP028047:5311591-5311908 [Phytobacter diazotrophicus]
ATGAGCCAGAATATCGATATCAGCAGCGGCAATGTTTATGCCGACATTGGACTGGACAATGCGGATGAAATGCAGGTTAA
AGCGCAGCTTGCTACCACCATTGGCAATATCATTAAAAGTCGTCGTCTTACTCAGGTGGAGGCGGCAAAGTTATTGGGGA
TGACACAGCCCAGGCTATCAAATATGCTGCGCGGCCAATTTCGCGGCATAAGTGAAGTAAAAATGCTTGAATGTCTGACA
CGCTTAGGGCGCGATGTTCAAATTGTGGTTGGGCGCCCGCGCCGTACCCCGGGACGTATGAAGGTGGTTTTTGCCTGA

Structures

Toxin

• AlphaFold DB: A0A348DL83

Antitoxin

• AlphaFold DB: A0A348DL84