Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 33225..33868 Replicon plasmid pTMTA97301
Accession NZ_AP028042
Organism Phytobacter diazotrophicus strain TA9730

Toxin (Protein)


Gene name vagD Uniprot ID A0A348DMH6
Locus tag QUE90_RS27115 Protein ID WP_047368852.1
Coordinates 33225..33641 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID A0A348DMH7
Locus tag QUE90_RS27120 Protein ID WP_047368851.1
Coordinates 33638..33868 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE90_RS27105 (PDTA9730_53460) 30000..30242 + 243 WP_047368854.1 hypothetical protein -
QUE90_RS27110 (PDTA9730_53470) 31809..33038 + 1230 WP_047368853.1 HDOD domain-containing protein -
QUE90_RS27115 (PDTA9730_53480) 33225..33641 - 417 WP_047368852.1 type II toxin-antitoxin system VapC family toxin Toxin
QUE90_RS27120 (PDTA9730_53490) 33638..33868 - 231 WP_047368851.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QUE90_RS27125 (PDTA9730_53500) 34213..34473 + 261 WP_007374405.1 type II toxin-antitoxin system ParD family antitoxin -
QUE90_RS27130 (PDTA9730_53510) 34460..34756 + 297 WP_047368850.1 type II toxin-antitoxin system RelE/ParE family toxin -
QUE90_RS27135 (PDTA9730_53520) 34986..35420 - 435 WP_007374421.1 hypothetical protein -
QUE90_RS27140 (PDTA9730_53530) 35638..37038 - 1401 WP_007374420.1 copper resistance membrane spanning protein PcoS -
QUE90_RS27145 (PDTA9730_53540) 37035..37715 - 681 WP_001188930.1 copper response regulator transcription factor PcoR -
QUE90_RS27150 (PDTA9730_53550) 37770..38699 - 930 WP_000998778.1 copper resistance inner membrane protein PcoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..175916 175916


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15042.32 Da        Isoelectric Point: 7.2449

>T44409 WP_047368852.1 NZ_AP028042:c33641-33225 [Phytobacter diazotrophicus]
VNKTYMLDTNICSLIMREQPEAVLKRLEQAVLRNHRTVISAITYQEMRSGATGPKASPRHIALVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAVAAGAILVTNNTREFERVPGLVLEDWVN

Download         Length: 417 bp

>T44409 NZ_AP028042:c33641-33225 [Phytobacter diazotrophicus]
GTGAACAAAACGTATATGCTGGACACTAACATCTGCTCATTAATCATGCGCGAGCAACCGGAAGCGGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCAACCACCGCACTGTGATCTCGGCCATCACCTACCAGGAGATGCGCTCCGGCGCCACGGGCC
CGAAGGCTTCGCCGCGCCATATTGCGCTGGTTGATGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCG
GCGGTCGACGCTACCACGGACATCCGGGTGGCGCTGCGCCTCGCAGGCACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCATGCGGTTGCCGCCGGCGCCATCCTGGTGACCAACAATACAAGAGAGTTTGAACGGGTTCCGGGTCTGGTGCTGG
AAGACTGGGTAAATTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8780.97 Da        Isoelectric Point: 4.6583

>AT44409 WP_047368851.1 NZ_AP028042:c33868-33638 [Phytobacter diazotrophicus]
MRTVSIFKNGNNRAIRLPRDMDFEGVSELEIVREGDRIILRPVRPTWGSFAKMEKADPDFMAEREDVVSDEGRVDL

Download         Length: 231 bp

>AT44409 NZ_AP028042:c33868-33638 [Phytobacter diazotrophicus]
ATGAGAACCGTATCTATTTTCAAAAACGGCAACAACCGCGCCATCCGTCTGCCCCGTGACATGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGAGACCGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGAAGATGG
AAAAAGCAGACCCGGACTTTATGGCGGAGCGTGAGGACGTTGTCAGTGACGAAGGACGGGTTGACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DMH6

Antitoxin

Source ID Structure
AlphaFold DB A0A348DMH7

References