Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4263928..4264547 Replicon chromosome
Accession NZ_AP028041
Organism Phytobacter diazotrophicus strain TA9730

Toxin (Protein)


Gene name Hha Uniprot ID A0A348DII6
Locus tag QUE90_RS20595 Protein ID WP_041852465.1
Coordinates 4264329..4264547 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A348DII5
Locus tag QUE90_RS20590 Protein ID WP_041852464.1
Coordinates 4263928..4264302 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE90_RS20580 (4259039) 4259039..4260235 + 1197 WP_041852462.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QUE90_RS20585 (4260258) 4260258..4263407 + 3150 WP_125125202.1 multidrug efflux RND transporter permease subunit AcrB -
QUE90_RS20590 (4263928) 4263928..4264302 + 375 WP_041852464.1 Hha toxicity modulator TomB Antitoxin
QUE90_RS20595 (4264329) 4264329..4264547 + 219 WP_041852465.1 HHA domain-containing protein Toxin
QUE90_RS20600 (4264616) 4264616..4265182 + 567 WP_125125203.1 maltose O-acetyltransferase -
QUE90_RS20605 (4265282) 4265282..4265749 + 468 WP_041852467.1 YlaC family protein -
QUE90_RS20610 (4265727) 4265727..4267172 - 1446 WP_125125204.1 PLP-dependent aminotransferase family protein -
QUE90_RS20615 (4267272) 4267272..4267973 + 702 WP_041852469.1 GNAT family protein -
QUE90_RS20620 (4267970) 4267970..4268110 - 141 WP_006818844.1 type B 50S ribosomal protein L36 -
QUE90_RS20625 (4268114) 4268114..4268374 - 261 WP_041852470.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8685.14 Da        Isoelectric Point: 8.9039

>T44407 WP_041852465.1 NZ_AP028041:4264329-4264547 [Phytobacter diazotrophicus]
MSDKPLTKIDYLMRLRRCQTIDTLERVIEKNKYELPDHELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T44407 NZ_AP028041:4264329-4264547 [Phytobacter diazotrophicus]
ATGTCTGATAAGCCATTAACCAAAATTGATTATTTGATGCGATTACGACGTTGTCAGACAATTGACACGCTCGAACGCGT
TATTGAAAAAAATAAATACGAACTGCCTGACCATGAACTGACGGTGTTTTATTCAGCAGCCGATCATCGTCTGGCTGAGC
TAACCATGAATAAACTGTATGACAAAATCCCTCCTTCTGTGTGGAAATTCGTACGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14600.44 Da        Isoelectric Point: 5.5072

>AT44407 WP_041852464.1 NZ_AP028041:4263928-4264302 [Phytobacter diazotrophicus]
MDEYSPKRHDIAQLKFLCETLYHDCLLNLEESHHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINTQDLQKWRKSGNRLFRCFVNVSKANPVSHSC

Download         Length: 375 bp

>AT44407 NZ_AP028041:4263928-4264302 [Phytobacter diazotrophicus]
ATGGACGAATACTCGCCAAAAAGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACGTTGTACCATGATTGTCTCCT
GAATCTTGAAGAAAGCCATCATGGATGGGTTAACGATCCTACTTCGGCAATCAATTTGCAGTTAAATGAGTTGATTGAAC
ATATAGCAACCTTCGCACTTAATTATAAAATTAAGTATAATGAAGATAATAAACTGATTGAGCAGATAGACGAATACCTT
GATGATACGTTTATGCTGTTCAGTAGCTACGGTATCAACACACAAGACTTGCAGAAATGGCGTAAATCGGGAAACCGATT
GTTCCGCTGTTTCGTCAATGTGAGCAAGGCTAACCCTGTTAGTCACTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A348DII6

Antitoxin

Source ID Structure
AlphaFold DB A0A348DII5

References