Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4133756..4134253 Replicon chromosome
Accession NZ_AP027978
Organism Escherichia coli strain VE-V11-M

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QQK36_RS20325 Protein ID WP_001173343.1
Coordinates 4133756..4133929 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag QQK36_RS20330 Protein ID WP_001425728.1
Coordinates 4133957..4134253 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK36_RS20315 (4130426) 4130426..4131883 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
QQK36_RS20320 (4132120) 4132120..4133637 + 1518 WP_001295074.1 lysine--tRNA ligase -
QQK36_RS20325 (4133756) 4133756..4133929 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QQK36_RS20330 (4133957) 4133957..4134253 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QQK36_RS20335 (4134480) 4134480..4134752 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QQK36_RS20340 (4134764) 4134764..4134994 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QQK36_RS20345 (4135175) 4135175..4136806 + 1632 WP_001216487.1 sensor histidine kinase -
QQK36_RS20350 (4136803) 4136803..4137522 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44384 WP_001173343.1 NZ_AP027978:c4133929-4133756 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44384 NZ_AP027978:c4133929-4133756 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT44384 WP_001425728.1 NZ_AP027978:c4134253-4133957 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44384 NZ_AP027978:c4134253-4133957 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References