Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3902980..3903556 Replicon chromosome
Accession NZ_AP027978
Organism Escherichia coli strain VE-V11-M

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QQK36_RS19145 Protein ID WP_001297643.1
Coordinates 3903269..3903556 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QQK36_RS19140 Protein ID WP_000063156.1
Coordinates 3902980..3903282 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK36_RS19125 (3899576) 3899576..3901726 + 2151 WP_001298933.1 pyruvate/proton symporter BtsT -
QQK36_RS19130 (3901776) 3901776..3901979 + 204 WP_000467859.1 YbdD/YjiX family protein -
QQK36_RS19135 (3901990) 3901990..3902946 + 957 WP_001295525.1 GTPase -
QQK36_RS19140 (3902980) 3902980..3903282 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QQK36_RS19145 (3903269) 3903269..3903556 - 288 WP_001297643.1 BrnT family toxin Toxin
QQK36_RS19150 (3903755) 3903755..3904452 - 698 Protein_3739 IS1 family transposase -
QQK36_RS19155 (3904509) 3904509..3904604 + 96 Protein_3740 dienelactone hydrolase family protein -
QQK36_RS19160 (3904750) 3904750..3905982 + 1233 WP_286611866.1 multidrug efflux MFS transporter MdtM -
QQK36_RS19165 (3906023) 3906023..3907303 + 1281 WP_001037377.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3903755..3903964 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T44383 WP_001297643.1 NZ_AP027978:c3903556-3903269 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T44383 NZ_AP027978:c3903556-3903269 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT44383 WP_000063156.1 NZ_AP027978:c3903282-3902980 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT44383 NZ_AP027978:c3903282-3902980 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References