Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3398749..3399367 Replicon chromosome
Accession NZ_AP027978
Organism Escherichia coli strain VE-V11-M

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QQK36_RS16745 Protein ID WP_001291435.1
Coordinates 3399149..3399367 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QQK36_RS16740 Protein ID WP_000344800.1
Coordinates 3398749..3399123 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK36_RS16730 (3393838) 3393838..3395031 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QQK36_RS16735 (3395054) 3395054..3398203 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QQK36_RS16740 (3398749) 3398749..3399123 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QQK36_RS16745 (3399149) 3399149..3399367 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QQK36_RS16750 (3399539) 3399539..3400090 + 552 WP_000102568.1 maltose O-acetyltransferase -
QQK36_RS16755 (3400206) 3400206..3400676 + 471 WP_000136192.1 YlaC family protein -
QQK36_RS16760 (3400840) 3400840..3402390 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QQK36_RS16765 (3402432) 3402432..3402785 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QQK36_RS16775 (3403164) 3403164..3403475 + 312 WP_000409911.1 MGMT family protein -
QQK36_RS16780 (3403506) 3403506..3404078 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44381 WP_001291435.1 NZ_AP027978:3399149-3399367 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44381 NZ_AP027978:3399149-3399367 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44381 WP_000344800.1 NZ_AP027978:3398749-3399123 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44381 NZ_AP027978:3398749-3399123 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References