Detailed information of TA system TA044365

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 28186..28778
• Replicon: plasmid pVE-V10-F_4
• Accession: NZ_AP027975
• Organism: Escherichia coli strain VE-V10-F

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: QQK73_RS25565
• Protein ID: WP_000520549.1
• Coordinates: 28530..28778 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: QQK73_RS25560
• Protein ID: WP_000681613.1
• Coordinates: 28186..28533 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK73_RS25530 (VEV10F_48960)	24191..24517	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
QQK73_RS25535 (VEV10F_48970)	24531..25151	-	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
QQK73_RS25540 (VEV10F_48990)	25507..25722	-	216	WP_000760375.1	EexN family lipoprotein	-
QQK73_RS25545 (VEV10F_49000)	25719..26012	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
QQK73_RS25550 (VEV10F_49010)	26089..27318	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
QQK73_RS25555 (VEV10F_49020)	27322..27825	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
QQK73_RS25560 (VEV10F_49030)	28186..28533	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QQK73_RS25565 (VEV10F_49040)	28530..28778	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
QQK73_RS25570 (VEV10F_49060)	29083..29433	-	351	WP_000854259.1	hypothetical protein	-
QQK73_RS25575 (VEV10F_49070)	29524..29823	-	300	WP_000650309.1	hypothetical protein	-
QQK73_RS25580 (VEV10F_49080)	30277..30504	-	228	WP_000801440.1	hypothetical protein	-
QQK73_RS25585 (VEV10F_49090)	30491..30742	-	252	WP_001230707.1	hypothetical protein	-
QQK73_RS25590 (VEV10F_49100)	30739..31281	-	543	WP_001083906.1	hypothetical protein	-
QQK73_RS25595 (VEV10F_49110)	31386..31901	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33309	33309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T44365 WP_000520549.1 NZ_AP027975:c28778-28530 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T44365 NZ_AP027975:c28778-28530 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT44365 WP_000681613.1 NZ_AP027975:c28533-28186 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT44365 NZ_AP027975:c28533-28186 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1