Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 141978..142621 Replicon plasmid pVE-V10-F_1
Accession NZ_AP027972
Organism Escherichia coli strain VE-V10-F

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag QQK73_RS24660 Protein ID WP_001034046.1
Coordinates 142205..142621 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag QQK73_RS24655 Protein ID WP_001261278.1
Coordinates 141978..142208 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK73_RS24625 (137488) 137488..137793 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
QQK73_RS24630 (137795) 137795..138013 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
QQK73_RS24635 (138605) 138605..139093 + 489 WP_011254646.1 hypothetical protein -
QQK73_RS24640 (139127) 139127..140260 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
QQK73_RS24645 (140427) 140427..141200 - 774 WP_000905949.1 hypothetical protein -
QQK73_RS24650 (141213) 141213..141713 - 501 WP_000528932.1 HEPN family nuclease -
QQK73_RS24655 (141978) 141978..142208 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QQK73_RS24660 (142205) 142205..142621 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
QQK73_RS24665 (142666) 142666..146460 - 3795 WP_001144731.1 hypothetical protein -
QQK73_RS24670 (146841) 146841..147071 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
QQK73_RS24675 (147068) 147068..147484 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD iroB / iroC / iroD / iroE / iroN / vat / iutA / iucD / iucC / iucB / iucA 1..169195 169195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T44360 WP_001034046.1 NZ_AP027972:142205-142621 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T44360 NZ_AP027972:142205-142621 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT44360 WP_001261278.1 NZ_AP027972:141978-142208 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT44360 NZ_AP027972:141978-142208 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References