Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4374974..4375471 Replicon chromosome
Accession NZ_AP027971
Organism Escherichia coli strain VE-V10-F

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QQK73_RS21470 Protein ID WP_001173343.1
Coordinates 4374974..4375147 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag QQK73_RS21475 Protein ID WP_001425728.1
Coordinates 4375175..4375471 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK73_RS21460 (4371644) 4371644..4373101 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
QQK73_RS21465 (4373338) 4373338..4374855 + 1518 WP_001295074.1 lysine--tRNA ligase -
QQK73_RS21470 (4374974) 4374974..4375147 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QQK73_RS21475 (4375175) 4375175..4375471 - 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QQK73_RS21480 (4375698) 4375698..4375970 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QQK73_RS21485 (4375982) 4375982..4376212 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QQK73_RS21490 (4376393) 4376393..4378024 + 1632 WP_001216487.1 sensor histidine kinase -
QQK73_RS21495 (4378021) 4378021..4378740 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44352 WP_001173343.1 NZ_AP027971:c4375147-4374974 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44352 NZ_AP027971:c4375147-4374974 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT44352 WP_001425728.1 NZ_AP027971:c4375471-4375175 [Escherichia coli]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44352 NZ_AP027971:c4375471-4375175 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References