Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 4270396..4270991 Replicon chromosome
Accession NZ_AP027971
Organism Escherichia coli strain VE-V10-F

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag QQK73_RS20940 Protein ID WP_000239579.1
Coordinates 4270396..4270746 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag QQK73_RS20945 Protein ID WP_001223208.1
Coordinates 4270740..4270991 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK73_RS20920 (4265672) 4265672..4266694 - 1023 WP_001339490.1 ABC transporter permease -
QQK73_RS20925 (4266708) 4266708..4268210 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
QQK73_RS20930 (4268520) 4268520..4269476 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
QQK73_RS20935 (4269786) 4269786..4270316 + 531 WP_000055072.1 inorganic diphosphatase -
QQK73_RS20940 (4270396) 4270396..4270746 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
QQK73_RS20945 (4270740) 4270740..4270991 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
QQK73_RS20950 (4271204) 4271204..4271545 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
QQK73_RS20955 (4271548) 4271548..4275327 - 3780 WP_000060921.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T44351 WP_000239579.1 NZ_AP027971:c4270746-4270396 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T44351 NZ_AP027971:c4270746-4270396 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT44351 WP_001223208.1 NZ_AP027971:c4270991-4270740 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT44351 NZ_AP027971:c4270991-4270740 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References