Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3583394..3584012 Replicon chromosome
Accession NZ_AP027971
Organism Escherichia coli strain VE-V10-F

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QQK73_RS17600 Protein ID WP_001291435.1
Coordinates 3583794..3584012 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QQK73_RS17595 Protein ID WP_000344800.1
Coordinates 3583394..3583768 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK73_RS17585 (3578483) 3578483..3579676 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QQK73_RS17590 (3579699) 3579699..3582848 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QQK73_RS17595 (3583394) 3583394..3583768 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QQK73_RS17600 (3583794) 3583794..3584012 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QQK73_RS17605 (3584184) 3584184..3584735 + 552 WP_000102568.1 maltose O-acetyltransferase -
QQK73_RS17610 (3584851) 3584851..3585321 + 471 WP_000136192.1 YlaC family protein -
QQK73_RS17615 (3585485) 3585485..3587035 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QQK73_RS17620 (3587077) 3587077..3587430 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QQK73_RS17630 (3587809) 3587809..3588120 + 312 WP_000409911.1 MGMT family protein -
QQK73_RS17635 (3588151) 3588151..3588723 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44349 WP_001291435.1 NZ_AP027971:3583794-3584012 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44349 NZ_AP027971:3583794-3584012 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44349 WP_000344800.1 NZ_AP027971:3583394-3583768 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44349 NZ_AP027971:3583394-3583768 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References