Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 263435..264021 Replicon chromosome
Accession NZ_AP027971
Organism Escherichia coli strain VE-V10-F

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag QQK73_RS01205 Protein ID WP_042014162.1
Coordinates 263435..263665 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QQK73_RS01210 Protein ID WP_000593555.1
Coordinates 263662..264021 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK73_RS01195 (259472) 259472..262207 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
QQK73_RS01200 (262207) 262207..263331 + 1125 WP_001314210.1 ABC transporter permease -
QQK73_RS01205 (263435) 263435..263665 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
QQK73_RS01210 (263662) 263662..264021 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QQK73_RS01215 (264141) 264141..264542 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QQK73_RS01220 (264548) 264548..265354 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QQK73_RS01225 (265351) 265351..266115 - 765 WP_174563175.1 nickel import ATP-binding protein NikD -
QQK73_RS01230 (266115) 266115..266948 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QQK73_RS01235 (266945) 266945..267889 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T44329 WP_042014162.1 NZ_AP027971:263435-263665 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T44329 NZ_AP027971:263435-263665 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT44329 WP_000593555.1 NZ_AP027971:263662-264021 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT44329 NZ_AP027971:263662-264021 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References