Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 19303..19946 Replicon plasmid pVE-T9_1
Accession NZ_AP027964
Organism Escherichia coli strain VE-T9

Toxin (Protein)


Gene name vagD Uniprot ID B1LRW4
Locus tag QQK52_RS22400 Protein ID WP_001044768.1
Coordinates 19303..19719 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D2WFK3
Locus tag QQK52_RS22405 Protein ID WP_001261287.1
Coordinates 19716..19946 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK52_RS22380 (VET9_43040) 14514..15539 - 1026 WP_286626598.1 AAA family ATPase -
QQK52_RS22385 (VET9_43050) 15802..16392 - 591 WP_000194575.1 hypothetical protein -
QQK52_RS22390 (VET9_43060) 16392..16649 - 258 WP_000343085.1 hypothetical protein -
QQK52_RS22395 (VET9_43070) 17003..19141 + 2139 WP_000350638.1 AAA family ATPase -
QQK52_RS22400 (VET9_43080) 19303..19719 - 417 WP_001044768.1 type II toxin-antitoxin system VapC family toxin Toxin
QQK52_RS22405 (VET9_43090) 19716..19946 - 231 WP_001261287.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QQK52_RS22410 (VET9_43100) 20242..20514 + 273 WP_061602529.1 hypothetical protein -
QQK52_RS22415 (VET9_43110) 20561..21265 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
QQK52_RS22420 (VET9_43120) 21312..21746 - 435 WP_000429836.1 Hg(II)-responsive transcriptional regulator -
QQK52_RS22425 (VET9_43130) 21818..22168 + 351 WP_001294663.1 mercuric transport protein MerT -
QQK52_RS22430 (VET9_43140) 22182..22457 + 276 WP_000732292.1 mercury resistance system periplasmic binding protein MerP -
QQK52_RS22435 (VET9_43150) 22493..22915 + 423 WP_001340589.1 organomercurial transporter MerC -
QQK52_RS22440 (VET9_43160) 22967..24661 + 1695 WP_000105636.1 mercury(II) reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(3')-Ia / mef(B) / sul3 / ant(3'')-Ia / cmlA1 / floR / dfrA14 / blaOXA-10 / ARR-3 / qnrS1 / tet(A) - 1..102149 102149
- flank IS/Tn - - 20549..21265 716


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15197.66 Da        Isoelectric Point: 7.7805

>T44325 WP_001044768.1 NZ_AP027964:c19719-19303 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDEFCARLDAILPWDRA
AVDATTKIKVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPDLVLEDWVK

Download         Length: 417 bp

>T44325 NZ_AP027964:c19719-19303 [Escherichia coli]
GTGAACAAGACTTATATGCTTGATACCTGTATCTGCTCGTTCATCATGCGTGAACAGCCAGAAGCCGTCCTGAAGCGTCT
GGAGCAGGCGGTGCTACGCGGCCACCGTATAGTGGTCTCGGCCATCACCTACTCTGAAATGCGCTTCGGGGCTACCGGCC
CAAAGGCCTCACCACGTCACGTCCAACTGGTCGATGAATTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCC
GCAGTAGACGCCACCACGAAGATTAAAGTGGCACTGCGGCTGGCCGGGACGCCGATCGGCCCGAACGACACGGCGATTGC
CGGGCACGCCATCGCCGCCGGGGCGATACTGGTAACGAATAATACAAGAGAATTTGAGCGAGTGCCTGACCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8815.90 Da        Isoelectric Point: 4.3775

>AT44325 WP_001261287.1 NZ_AP027964:c19946-19716 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLEYEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT44325 NZ_AP027964:c19946-19716 [Escherichia coli]
ATGAGAACCGTATCGATATTCAAAAATGGCAACAACCGCGCCATCCGTCTGCCTCGTGATCTGGATTTTGAGGGGGTGAG
CGAGCTCGAGATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCCGTCCGGCCAACCTGGGGATCATTCCTAGAGTACG
AAAAAGCCGATCCAGATTTTATGGCGGAGCGCGAGGATGTTGTCAGCGACGAAGGGCGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A606Q844

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QFC4

References