Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3922165..3922741 Replicon chromosome
Accession NZ_AP027963
Organism Escherichia coli strain VE-T9

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QQK52_RS19195 Protein ID WP_001297643.1
Coordinates 3922454..3922741 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QQK52_RS19190 Protein ID WP_000063156.1
Coordinates 3922165..3922467 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK52_RS19175 (3918761) 3918761..3920911 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QQK52_RS19180 (3920961) 3920961..3921164 + 204 WP_000467859.1 YbdD/YjiX family protein -
QQK52_RS19185 (3921175) 3921175..3922131 + 957 WP_001297640.1 GTPase -
QQK52_RS19190 (3922165) 3922165..3922467 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QQK52_RS19195 (3922454) 3922454..3922741 - 288 WP_001297643.1 BrnT family toxin Toxin
QQK52_RS19200 (3922940) 3922940..3923637 - 698 Protein_3756 IS1 family transposase -
QQK52_RS19205 (3923694) 3923694..3923789 + 96 Protein_3757 dienelactone hydrolase family protein -
QQK52_RS19210 (3923935) 3923935..3925167 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QQK52_RS19215 (3925208) 3925208..3926488 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3922940..3923149 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T44323 WP_001297643.1 NZ_AP027963:c3922741-3922454 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T44323 NZ_AP027963:c3922741-3922454 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT44323 WP_000063156.1 NZ_AP027963:c3922467-3922165 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT44323 NZ_AP027963:c3922467-3922165 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References