Detailed information of TA system TA044320

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3391704..3392541
• Replicon: chromosome
• Accession: NZ_AP027963
• Organism: Escherichia coli strain VE-T9

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QQK52_RS16520
• Protein ID: WP_000227784.1
• Coordinates: 3391999..3392541 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QQK52_RS16515
• Protein ID: WP_001297137.1
• Coordinates: 3391704..3392015 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK52_RS16490 (3386724)	3386724..3387671	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
QQK52_RS16495 (3387693)	3387693..3389684	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QQK52_RS16500 (3389674)	3389674..3390288	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QQK52_RS16505 (3390288)	3390288..3390617	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QQK52_RS16510 (3390629)	3390629..3391519	+	891	WP_097346802.1	heme o synthase	-
QQK52_RS16515 (3391704)	3391704..3392015	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QQK52_RS16520 (3391999)	3391999..3392541	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QQK52_RS16525 (3392597)	3392597..3393532	-	936	WP_001490882.1	tetratricopeptide repeat protein	-
QQK52_RS16530 (3393940)	3393940..3395304	+	1365	WP_001000954.1	MFS transporter	-
QQK52_RS16535 (3395432)	3395432..3395923	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
QQK52_RS16540 (3396091)	3396091..3397002	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T44320 WP_000227784.1 NZ_AP027963:3391999-3392541 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T44320 NZ_AP027963:3391999-3392541 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT44320 WP_001297137.1 NZ_AP027963:3391704-3392015 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT44320 NZ_AP027963:3391704-3392015 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3