Detailed information of TA system TA044306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 941761..942344
• Replicon: chromosome
• Accession: NZ_AP027963
• Organism: Escherichia coli strain VE-T9

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QQK52_RS04665
• Protein ID: WP_089601808.1
• Coordinates: 942009..942344 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A161R357
• Locus tag: QQK52_RS04660
• Protein ID: WP_040062758.1
• Coordinates: 941761..942009 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK52_RS04650 (938100)	938100..939401	+	1302	WP_000046826.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
QQK52_RS04655 (939449)	939449..941683	+	2235	WP_000226803.1	GTP diphosphokinase	-
QQK52_RS04660 (941761)	941761..942009	+	249	WP_040062758.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
QQK52_RS04665 (942009)	942009..942344	+	336	WP_089601808.1	endoribonuclease MazF	Toxin
QQK52_RS04670 (942415)	942415..943206	+	792	WP_001071643.1	nucleoside triphosphate pyrophosphohydrolase	-
QQK52_RS04675 (943434)	943434..945071	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
QQK52_RS04680 (945159)	945159..946457	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
QQK52_RS04685 (946601)	946601..946794	+	194	Protein_917	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• Antitoxin PemI-like (2-82)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12142.09 Da
• Isoelectric Point: 8.2618

>T44306 WP_089601808.1 NZ_AP027963:942009-942344 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKSSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTLAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T44306 NZ_AP027963:942009-942344 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAAGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCTTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACACTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9297.70 Da
• Isoelectric Point: 4.5972

>AT44306 WP_040062758.1 NZ_AP027963:941761-942009 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNGITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT44306 NZ_AP027963:941761-942009 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATTCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGGCATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A161R357