Detailed information of TA system TA044303

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 28186..28778
• Replicon: plasmid pVE-T2_2
• Accession: NZ_AP027962
• Organism: Escherichia coli strain VE-T2

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: QQK13_RS24445
• Protein ID: WP_000520549.1
• Coordinates: 28530..28778 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: QQK13_RS24440
• Protein ID: WP_000681613.1
• Coordinates: 28186..28533 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK13_RS24410 (VET2_46810)	24191..24517	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
QQK13_RS24415 (VET2_46820)	24531..25151	-	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
QQK13_RS24420 (VET2_46840)	25507..25722	-	216	WP_000760375.1	EexN family lipoprotein	-
QQK13_RS24425 (VET2_46850)	25719..26012	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
QQK13_RS24430 (VET2_46860)	26089..27318	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
QQK13_RS24435 (VET2_46870)	27322..27825	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
QQK13_RS24440 (VET2_46880)	28186..28533	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QQK13_RS24445 (VET2_46890)	28530..28778	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
QQK13_RS24450 (VET2_46910)	29083..29433	-	351	WP_000854259.1	hypothetical protein	-
QQK13_RS24455 (VET2_46920)	29524..29823	-	300	WP_000650309.1	hypothetical protein	-
QQK13_RS24460 (VET2_46930)	30277..30504	-	228	WP_000801440.1	hypothetical protein	-
QQK13_RS24465 (VET2_46940)	30491..30742	-	252	WP_001230707.1	hypothetical protein	-
QQK13_RS24470 (VET2_46950)	30739..31281	-	543	WP_001083906.1	hypothetical protein	-
QQK13_RS24475 (VET2_46960)	31386..31901	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33309	33309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T44303 WP_000520549.1 NZ_AP027962:c28778-28530 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T44303 NZ_AP027962:c28778-28530 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT44303 WP_000681613.1 NZ_AP027962:c28533-28186 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT44303 NZ_AP027962:c28533-28186 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1