Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 61171..61814 Replicon plasmid pVE-T2_1
Accession NZ_AP027961
Organism Escherichia coli strain VE-T2

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag QQK13_RS24165 Protein ID WP_001034046.1
Coordinates 61398..61814 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag QQK13_RS24160 Protein ID WP_001261278.1
Coordinates 61171..61401 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK13_RS24130 (56681) 56681..56986 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -
QQK13_RS24135 (56988) 56988..57206 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
QQK13_RS24140 (57774) 57774..58286 + 513 WP_000151784.1 hypothetical protein -
QQK13_RS24145 (58320) 58320..59453 - 1134 WP_000545984.1 DUF3800 domain-containing protein -
QQK13_RS24150 (59620) 59620..60393 - 774 WP_000905949.1 hypothetical protein -
QQK13_RS24155 (60406) 60406..60906 - 501 WP_000528932.1 HEPN family nuclease -
QQK13_RS24160 (61171) 61171..61401 + 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QQK13_RS24165 (61398) 61398..61814 + 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
QQK13_RS24170 (61859) 61859..65653 - 3795 WP_001144731.1 hypothetical protein -
QQK13_RS24175 (66034) 66034..66264 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
QQK13_RS24180 (66261) 66261..66677 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / aac(3)-IId / sitABCD iutA / iucD / iucC / iucB / iucA 1..88388 88388


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T44301 WP_001034046.1 NZ_AP027961:61398-61814 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T44301 NZ_AP027961:61398-61814 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT44301 WP_001261278.1 NZ_AP027961:61171-61401 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT44301 NZ_AP027961:61171-61401 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References