Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4413395..4413892 Replicon chromosome
Accession NZ_AP027960
Organism Escherichia coli strain VE-T2

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QQK13_RS21580 Protein ID WP_001173343.1
Coordinates 4413395..4413568 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QQK13_RS21585 Protein ID WP_000398619.1
Coordinates 4413596..4413892 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK13_RS21570 (4410065) 4410065..4411522 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
QQK13_RS21575 (4411759) 4411759..4413276 + 1518 WP_001295074.1 lysine--tRNA ligase -
QQK13_RS21580 (4413395) 4413395..4413568 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QQK13_RS21585 (4413596) 4413596..4413892 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QQK13_RS21590 (4414119) 4414119..4414391 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
QQK13_RS21595 (4414403) 4414403..4414633 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QQK13_RS21600 (4414814) 4414814..4416445 + 1632 WP_001216487.1 sensor histidine kinase -
QQK13_RS21605 (4416442) 4416442..4417161 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-55 papB / papI 4335433..4413892 78459


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44293 WP_001173343.1 NZ_AP027960:c4413568-4413395 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44293 NZ_AP027960:c4413568-4413395 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT44293 WP_000398619.1 NZ_AP027960:c4413892-4413596 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44293 NZ_AP027960:c4413892-4413596 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References