Detailed information of TA system TA044290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3607731..3608568
• Replicon: chromosome
• Accession: NZ_AP027960
• Organism: Escherichia coli strain VE-T2

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QQK13_RS17655
• Protein ID: WP_000227784.1
• Coordinates: 3608026..3608568 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QQK13_RS17650
• Protein ID: WP_001297137.1
• Coordinates: 3607731..3608042 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK13_RS17625 (3602751)	3602751..3603698	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
QQK13_RS17630 (3603720)	3603720..3605711	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QQK13_RS17635 (3605701)	3605701..3606315	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QQK13_RS17640 (3606315)	3606315..3606644	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QQK13_RS17645 (3606656)	3606656..3607546	+	891	WP_000971336.1	heme o synthase	-
QQK13_RS17650 (3607731)	3607731..3608042	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QQK13_RS17655 (3608026)	3608026..3608568	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QQK13_RS17660 (3608624)	3608624..3609559	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
QQK13_RS17665 (3609967)	3609967..3611331	+	1365	WP_001000974.1	MFS transporter	-
QQK13_RS17670 (3611459)	3611459..3611950	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
QQK13_RS17675 (3612118)	3612118..3613029	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T44290 WP_000227784.1 NZ_AP027960:3608026-3608568 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T44290 NZ_AP027960:3608026-3608568 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT44290 WP_001297137.1 NZ_AP027960:3607731-3608042 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT44290 NZ_AP027960:3607731-3608042 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3