Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2543316..2543954 Replicon chromosome
Accession NZ_AP027960
Organism Escherichia coli strain VE-T2

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QQK13_RS12385 Protein ID WP_000813794.1
Coordinates 2543778..2543954 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QQK13_RS12380 Protein ID WP_001270286.1
Coordinates 2543316..2543732 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK13_RS12360 (2538468) 2538468..2539409 - 942 WP_001251315.1 ABC transporter permease -
QQK13_RS12365 (2539410) 2539410..2540423 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
QQK13_RS12370 (2540441) 2540441..2541586 - 1146 WP_106464763.1 ABC transporter substrate-binding protein -
QQK13_RS12375 (2541831) 2541831..2543237 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
QQK13_RS12380 (2543316) 2543316..2543732 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QQK13_RS12385 (2543778) 2543778..2543954 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QQK13_RS12390 (2544176) 2544176..2544406 + 231 WP_000494244.1 YncJ family protein -
QQK13_RS12395 (2544498) 2544498..2546459 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QQK13_RS12400 (2546532) 2546532..2547068 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QQK13_RS12405 (2547160) 2547160..2548335 + 1176 WP_001236215.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T44288 WP_000813794.1 NZ_AP027960:c2543954-2543778 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T44288 NZ_AP027960:c2543954-2543778 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT44288 WP_001270286.1 NZ_AP027960:c2543732-2543316 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT44288 NZ_AP027960:c2543732-2543316 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References