Detailed information of TA system TA044278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1353673..1354298
• Replicon: chromosome
• Accession: NZ_AP027960
• Organism: Escherichia coli strain VE-T2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QQK13_RS06570
• Protein ID: WP_000911330.1
• Coordinates: 1353900..1354298 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QQK13_RS06565
• Protein ID: WP_000450524.1
• Coordinates: 1353673..1353900 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK13_RS06540 (1349476)	1349476..1349946	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
QQK13_RS06545 (1349946)	1349946..1350518	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QQK13_RS06550 (1350664)	1350664..1351542	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QQK13_RS06555 (1351559)	1351559..1352593	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
QQK13_RS06560 (1352806)	1352806..1353519	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QQK13_RS06565 (1353673)	1353673..1353900	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QQK13_RS06570 (1353900)	1353900..1354298	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QQK13_RS06575 (1354445)	1354445..1355308	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
QQK13_RS06580 (1355323)	1355323..1357338	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
QQK13_RS06585 (1357412)	1357412..1358110	+	699	WP_000679812.1	esterase	-
QQK13_RS06590 (1358191)	1358191..1358391	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T44278 WP_000911330.1 NZ_AP027960:1353900-1354298 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T44278 NZ_AP027960:1353900-1354298 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT44278 WP_000450524.1 NZ_AP027960:1353673-1353900 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT44278 NZ_AP027960:1353673-1353900 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33