Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 352505..353136 Replicon chromosome
Accession NZ_AP027960
Organism Escherichia coli strain VE-T2

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag QQK13_RS01630 Protein ID WP_001259388.1
Coordinates 352505..352780 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QQK13_RS01635 Protein ID WP_000593555.1
Coordinates 352777..353136 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK13_RS01615 (347509) 347509..348576 + 1068 WP_000361485.1 HlyD family secretion protein -
QQK13_RS01620 (348573) 348573..351308 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
QQK13_RS01625 (351308) 351308..352432 + 1125 WP_001314210.1 ABC transporter permease -
QQK13_RS01630 (352505) 352505..352780 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
QQK13_RS01635 (352777) 352777..353136 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QQK13_RS01640 (353256) 353256..353657 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QQK13_RS01645 (353663) 353663..354469 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QQK13_RS01650 (354466) 354466..355230 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QQK13_RS01655 (355230) 355230..356063 - 834 WP_106464827.1 nickel ABC transporter permease subunit NikC -
QQK13_RS01660 (356060) 356060..357004 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 337476..353136 15660


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T44274 WP_001259388.1 NZ_AP027960:352505-352780 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T44274 NZ_AP027960:352505-352780 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT44274 WP_000593555.1 NZ_AP027960:352777-353136 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT44274 NZ_AP027960:352777-353136 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References