Detailed information of TA system TA044272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 28185..28777
• Replicon: plasmid pVE-T10_2
• Accession: NZ_AP027959
• Organism: Escherichia coli strain VE-T10

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: V0AGQ2
• Locus tag: QQK14_RS24415
• Protein ID: WP_000520549.1
• Coordinates: 28529..28777 (-)
• Length: 83 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: V0AWG0
• Locus tag: QQK14_RS24410
• Protein ID: WP_000681613.1
• Coordinates: 28185..28532 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK14_RS24380 (VET10_46810)	24190..24516	-	327	WP_000008647.1	TrbC/VirB2 family protein	-
QQK14_RS24385 (VET10_46820)	24530..25150	-	621	WP_000157549.1	lytic transglycosylase domain-containing protein	-
QQK14_RS24390 (VET10_46840)	25506..25721	-	216	WP_000760375.1	EexN family lipoprotein	-
QQK14_RS24395 (VET10_46850)	25718..26011	-	294	WP_060598542.1	sigma factor-like helix-turn-helix DNA-binding protein	-
QQK14_RS24400 (VET10_46860)	26088..27317	-	1230	WP_001329504.1	DNA relaxase/nickase TaxC	-
QQK14_RS24405 (VET10_46870)	27321..27824	-	504	WP_000781813.1	relaxosome-coupling protein TaxA	-
QQK14_RS24410 (VET10_46880)	28185..28532	-	348	WP_000681613.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QQK14_RS24415 (VET10_46890)	28529..28777	-	249	WP_000520549.1	type II toxin-antitoxin system HicA family toxin	Toxin
QQK14_RS24420 (VET10_46910)	29082..29432	-	351	WP_000854259.1	hypothetical protein	-
QQK14_RS24425 (VET10_46920)	29523..29822	-	300	WP_000650309.1	hypothetical protein	-
QQK14_RS24430 (VET10_46930)	30276..30503	-	228	WP_000801440.1	hypothetical protein	-
QQK14_RS24435 (VET10_46940)	30490..30741	-	252	WP_001230707.1	hypothetical protein	-
QQK14_RS24440 (VET10_46950)	30738..31280	-	543	WP_001083906.1	hypothetical protein	-
QQK14_RS24445 (VET10_46960)	31385..31900	-	516	WP_001025395.1	J domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-1.1	-	1..33308	33308

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-81)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 83 a.a.
• Molecular weight: 9422.08 Da
• Isoelectric Point: 10.4800

>T44272 WP_000520549.1 NZ_AP027959:c28777-28529 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Nucleotide

• Length: 249 bp

>T44272 NZ_AP027959:c28777-28529 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13113.84 Da
• Isoelectric Point: 4.6723

>AT44272 WP_000681613.1 NZ_AP027959:c28532-28185 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Nucleotide

• Length: 348 bp

>AT44272 NZ_AP027959:c28532-28185 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829CIX3

Antitoxin

• AlphaFold DB: A0A829CLC1