Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 73986..74629 Replicon plasmid pVE-T10_1
Accession NZ_AP027958
Organism Escherichia coli strain VE-T10

Toxin (Protein)


Gene name vagD Uniprot ID C7S9Y5
Locus tag QQK14_RS24200 Protein ID WP_001034046.1
Coordinates 73986..74402 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID V0SR71
Locus tag QQK14_RS24205 Protein ID WP_001261278.1
Coordinates 74399..74629 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK14_RS24185 (69123) 69123..69539 - 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
QQK14_RS24190 (69536) 69536..69766 - 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
QQK14_RS24195 (70147) 70147..73941 + 3795 WP_001144731.1 hypothetical protein -
QQK14_RS24200 (73986) 73986..74402 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin Toxin
QQK14_RS24205 (74399) 74399..74629 - 231 WP_001261278.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QQK14_RS24210 (74894) 74894..75394 + 501 WP_000528932.1 HEPN family nuclease -
QQK14_RS24215 (75407) 75407..76180 + 774 WP_000905949.1 hypothetical protein -
QQK14_RS24220 (76347) 76347..77480 + 1134 WP_000545984.1 DUF3800 domain-containing protein -
QQK14_RS24225 (77514) 77514..78026 - 513 WP_000151784.1 hypothetical protein -
QQK14_RS24230 (78594) 78594..78812 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA -
QQK14_RS24235 (78814) 78814..79119 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sitABCD iucA / iucB / iucC / iucD / iutA 1..81505 81505


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-53)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 14978.31 Da        Isoelectric Point: 6.7113

>T44271 WP_001034046.1 NZ_AP027958:c74402-73986 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEAVLKNLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEVKVALRLAGTPIGPNDTAIAGHAIATGAILVTNNVREFERVPGLVLEDWAG

Download         Length: 417 bp

>T44271 NZ_AP027958:c74402-73986 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCTGAAGCAGTGCTTAAGAATCT
GGAGCAGGCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGTC
CAAAGGCATCGCCACGCCACGTGCAGCTGGTTGACGCGTTCTGCGCCCGTCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGATGCAACCACGGAGGTTAAGGTGGCGCTGCGCCTCGCCGGCACGCCGATCGGTCCAAACGACACGGCGATCGC
CGGGCACGCCATCGCCACCGGGGCAATACTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTGCCTGGACTGGTACTGG
AAGACTGGGCAGGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8853.07 Da        Isoelectric Point: 4.6437

>AT44271 WP_001261278.1 NZ_AP027958:c74629-74399 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDRIILRPLRPSWSSFLELEKADPGFMTEREDVVCDEGRFNL

Download         Length: 231 bp

>AT44271 NZ_AP027958:c74629-74399 [Escherichia coli]
ATGAGAACCGTATCTATTTTCAAAAATGGCAATAACCGCGCGATCCGTCTGCCCCGCGATCTGGATTTTGAGGGGGTGAG
TGAGCTGGAGATTGTACGGGAAGGGGACAGAATCATTCTGCGTCCCCTCCGCCCGAGCTGGAGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGGCTTTATGACGGAACGCGAGGACGTTGTCTGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9NXF9

Antitoxin

Source ID Structure
AlphaFold DB V0SR71

References