Detailed information of TA system TA044269

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 16851..17386
• Replicon: plasmid pVE-T10_1
• Accession: NZ_AP027958
• Organism: Escherichia coli strain VE-T10

Toxin (Protein)

• Gene name: relE
• Uniprot ID: Q3YTD6
• Locus tag: QQK14_RS23905
• Protein ID: WP_000222766.1
• Coordinates: 17099..17386 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0VIP2
• Locus tag: QQK14_RS23900
• Protein ID: WP_001132900.1
• Coordinates: 16851..17102 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK14_RS23865 (12032)	12032..12646	+	615	Protein_16	conjugal transfer pilus acetylase TraX	-
QQK14_RS23870 (12701)	12701..13261	+	561	WP_000139310.1	fertility inhibition protein FinO	-
QQK14_RS23875 (13396)	13396..13608	+	213	WP_001541896.1	ANR family transcriptional regulator	-
- (13950)	13950..14011	-	62	NuclAT_0	-	-
- (13950)	13950..14011	-	62	NuclAT_0	-	-
- (13950)	13950..14011	-	62	NuclAT_0	-	-
- (13950)	13950..14011	-	62	NuclAT_0	-	-
QQK14_RS23880 (14055)	14055..14204	+	150	WP_001312851.1	Hok/Gef family protein	-
QQK14_RS23885 (14472)	14472..14729	+	258	WP_001541895.1	replication regulatory protein RepA	-
QQK14_RS23890 (14961)	14961..15035	+	75	WP_061357229.1	RepA leader peptide Tap	-
QQK14_RS23895 (15028)	15028..15885	+	858	WP_039002220.1	incFII family plasmid replication initiator RepA	-
QQK14_RS23900 (16851)	16851..17102	+	252	WP_001132900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QQK14_RS23905 (17099)	17099..17386	+	288	WP_000222766.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QQK14_RS23910 (17461)	17461..17649	-	189	Protein_25	IS3 family transposase	-
QQK14_RS23915 (17683)	17683..19196	-	1514	Protein_26	Tn3-like element TnAs1 family transposase	-
QQK14_RS23920 (19262)	19262..19963	+	702	Protein_27	IS6-like element IS26 family transposase	-
QQK14_RS23925 (20211)	20211..20498	+	288	WP_230071785.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	sul2 / floR / dfrA12 / aadA2 / cmlA1 / ant(3'')-Ia / tet(M) / sitABCD	iucA / iucB / iucC / iucD / iutA	1..81505	81505

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11147.23 Da
• Isoelectric Point: 10.5832

>T44269 WP_000222766.1 NZ_AP027958:17099-17386 [Escherichia coli]
MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYNLASERMR

Nucleotide

• Length: 288 bp

>T44269 NZ_AP027958:17099-17386 [Escherichia coli]
ATGACTTATACGGTAAAATTCAGGGATGATGCGCTCAAGGAATGGCTGAAACTGGATAAGACCATTCAACAACAGTTTGT
GAAAAAACTAAAAAAATGCAGCGAAAATCCTCATATACCGTCAGCAAAACTAAGAGGGCTAAAGGATTGCTACAAAATTA
AACTACGTGCATCGGGATTTCGTCTGGTTTATCAGGTGATTGACGATATGTTAATTATTGCGGTTGTTGCGGTAGGAAAA
CGAGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9211.41 Da
• Isoelectric Point: 4.0780

>AT44269 WP_001132900.1 NZ_AP027958:16851-17102 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSNQPLHDVDLD
SYL

Nucleotide

• Length: 252 bp

>AT44269 NZ_AP027958:16851-17102 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTACCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCTGAACTGTATGAAAGGATGC
TTGATGCTCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTAGAC
AGTTATTTATGA

Structures

Toxin

• AlphaFold DB: A0A829CDZ2

Antitoxin

• AlphaFold DB: V0VIP2