Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4411752..4412249 Replicon chromosome
Accession NZ_AP027957
Organism Escherichia coli strain VE-T10

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QQK14_RS21585 Protein ID WP_001173343.1
Coordinates 4411752..4411925 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QQK14_RS21590 Protein ID WP_000398619.1
Coordinates 4411953..4412249 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK14_RS21575 (4408422) 4408422..4409879 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
QQK14_RS21580 (4410116) 4410116..4411633 + 1518 WP_001295074.1 lysine--tRNA ligase -
QQK14_RS21585 (4411752) 4411752..4411925 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QQK14_RS21590 (4411953) 4411953..4412249 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QQK14_RS21595 (4412476) 4412476..4412748 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
QQK14_RS21600 (4412760) 4412760..4412990 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QQK14_RS21605 (4413171) 4413171..4414802 + 1632 WP_001216487.1 sensor histidine kinase -
QQK14_RS21610 (4414799) 4414799..4415518 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-55 papB / papI 4331684..4412249 80565


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44263 WP_001173343.1 NZ_AP027957:c4411925-4411752 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44263 NZ_AP027957:c4411925-4411752 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT44263 WP_000398619.1 NZ_AP027957:c4412249-4411953 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44263 NZ_AP027957:c4412249-4411953 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References