Detailed information of TA system TA044258

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3607370..3608207
• Replicon: chromosome
• Accession: NZ_AP027957
• Organism: Escherichia coli strain VE-T10

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QQK14_RS17670
• Protein ID: WP_000227784.1
• Coordinates: 3607665..3608207 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QQK14_RS17665
• Protein ID: WP_001297137.1
• Coordinates: 3607370..3607681 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK14_RS17640 (3602390)	3602390..3603337	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
QQK14_RS17645 (3603359)	3603359..3605350	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QQK14_RS17650 (3605340)	3605340..3605954	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QQK14_RS17655 (3605954)	3605954..3606283	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QQK14_RS17660 (3606295)	3606295..3607185	+	891	WP_000971336.1	heme o synthase	-
QQK14_RS17665 (3607370)	3607370..3607681	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QQK14_RS17670 (3607665)	3607665..3608207	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QQK14_RS17675 (3608263)	3608263..3609198	-	936	WP_001365761.1	tetratricopeptide repeat protein	-
QQK14_RS17680 (3609606)	3609606..3610970	+	1365	WP_001000974.1	MFS transporter	-
QQK14_RS17685 (3611098)	3611098..3611589	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
QQK14_RS17690 (3611757)	3611757..3612668	+	912	WP_000705874.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T44258 WP_000227784.1 NZ_AP027957:3607665-3608207 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T44258 NZ_AP027957:3607665-3608207 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT44258 WP_001297137.1 NZ_AP027957:3607370-3607681 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT44258 NZ_AP027957:3607370-3607681 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3