Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2543128..2543766 Replicon chromosome
Accession NZ_AP027957
Organism Escherichia coli strain VE-T10

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QQK14_RS12400 Protein ID WP_000813794.1
Coordinates 2543590..2543766 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QQK14_RS12395 Protein ID WP_001270286.1
Coordinates 2543128..2543544 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK14_RS12375 (2538280) 2538280..2539221 - 942 WP_001251315.1 ABC transporter permease -
QQK14_RS12380 (2539222) 2539222..2540235 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
QQK14_RS12385 (2540253) 2540253..2541398 - 1146 WP_106464763.1 ABC transporter substrate-binding protein -
QQK14_RS12390 (2541643) 2541643..2543049 - 1407 WP_000760592.1 PLP-dependent aminotransferase family protein -
QQK14_RS12395 (2543128) 2543128..2543544 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QQK14_RS12400 (2543590) 2543590..2543766 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QQK14_RS12405 (2543988) 2543988..2544218 + 231 WP_000494244.1 YncJ family protein -
QQK14_RS12410 (2544310) 2544310..2546271 - 1962 WP_001593323.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QQK14_RS12415 (2546344) 2546344..2546880 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QQK14_RS12420 (2546933) 2546933..2548147 + 1215 WP_001348055.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T44255 WP_000813794.1 NZ_AP027957:c2543766-2543590 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T44255 NZ_AP027957:c2543766-2543590 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT44255 WP_001270286.1 NZ_AP027957:c2543544-2543128 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT44255 NZ_AP027957:c2543544-2543128 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References