Detailed information of TA system TA044245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1353475..1354100
• Replicon: chromosome
• Accession: NZ_AP027957
• Organism: Escherichia coli strain VE-T10

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QQK14_RS06570
• Protein ID: WP_000911330.1
• Coordinates: 1353702..1354100 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QQK14_RS06565
• Protein ID: WP_000450524.1
• Coordinates: 1353475..1353702 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QQK14_RS06540 (1349278)	1349278..1349748	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
QQK14_RS06545 (1349748)	1349748..1350320	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
QQK14_RS06550 (1350466)	1350466..1351344	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QQK14_RS06555 (1351361)	1351361..1352395	+	1035	WP_001330579.1	outer membrane protein assembly factor BamC	-
QQK14_RS06560 (1352608)	1352608..1353321	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QQK14_RS06565 (1353475)	1353475..1353702	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QQK14_RS06570 (1353702)	1353702..1354100	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QQK14_RS06575 (1354247)	1354247..1355110	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
QQK14_RS06580 (1355125)	1355125..1357140	+	2016	WP_000829292.1	tRNA cytosine(34) acetyltransferase TmcA	-
QQK14_RS06585 (1357214)	1357214..1357912	+	699	WP_000679812.1	esterase	-
QQK14_RS06590 (1357993)	1357993..1358193	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T44245 WP_000911330.1 NZ_AP027957:1353702-1354100 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T44245 NZ_AP027957:1353702-1354100 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT44245 WP_000450524.1 NZ_AP027957:1353475-1353702 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT44245 NZ_AP027957:1353475-1353702 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33