Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 924137..924791 Replicon chromosome
Accession NZ_AP027957
Organism Escherichia coli strain VE-T10

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag QQK14_RS04530 Protein ID WP_000244772.1
Coordinates 924384..924791 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QQK14_RS04525 Protein ID WP_000354046.1
Coordinates 924137..924403 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK14_RS04500 (919425) 919425..920168 + 744 WP_000951964.1 SDR family oxidoreductase -
QQK14_RS04505 (920225) 920225..921658 - 1434 WP_001344773.1 6-phospho-beta-glucosidase BglA -
QQK14_RS04510 (921703) 921703..922014 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QQK14_RS04515 (922178) 922178..922837 + 660 WP_000250274.1 hemolysin III family protein -
QQK14_RS04520 (922914) 922914..923894 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QQK14_RS04525 (924137) 924137..924403 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QQK14_RS04530 (924384) 924384..924791 + 408 WP_000244772.1 protein YgfX Toxin
QQK14_RS04535 (924831) 924831..925352 - 522 WP_001055874.1 flavodoxin FldB -
QQK14_RS04540 (925464) 925464..926360 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QQK14_RS04545 (926385) 926385..927095 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QQK14_RS04550 (927101) 927101..928834 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T44243 WP_000244772.1 NZ_AP027957:924384-924791 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T44243 NZ_AP027957:924384-924791 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT44243 WP_000354046.1 NZ_AP027957:924137-924403 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT44243 NZ_AP027957:924137-924403 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References