Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 352400..353031 Replicon chromosome
Accession NZ_AP027957
Organism Escherichia coli strain VE-T10

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag QQK14_RS01635 Protein ID WP_001259388.1
Coordinates 352400..352675 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QQK14_RS01640 Protein ID WP_000593555.1
Coordinates 352672..353031 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK14_RS01620 (347404) 347404..348471 + 1068 WP_000361485.1 HlyD family secretion protein -
QQK14_RS01625 (348468) 348468..351203 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
QQK14_RS01630 (351203) 351203..352327 + 1125 WP_001314210.1 ABC transporter permease -
QQK14_RS01635 (352400) 352400..352675 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
QQK14_RS01640 (352672) 352672..353031 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QQK14_RS01645 (353151) 353151..353552 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QQK14_RS01650 (353558) 353558..354364 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QQK14_RS01655 (354361) 354361..355125 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QQK14_RS01660 (355125) 355125..355958 - 834 WP_106464827.1 nickel ABC transporter permease subunit NikC -
QQK14_RS01665 (355955) 355955..356899 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T44241 WP_001259388.1 NZ_AP027957:352400-352675 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T44241 NZ_AP027957:352400-352675 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT44241 WP_000593555.1 NZ_AP027957:352672-353031 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT44241 NZ_AP027957:352672-353031 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References