Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | hicAB/- |
Location | 28186..28778 | Replicon | plasmid pVE-F5_2 |
Accession | NZ_AP027955 | ||
Organism | Escherichia coli strain VE-F5 |
Toxin (Protein)
Gene name | hicA | Uniprot ID | V0AGQ2 |
Locus tag | QQJ99_RS24085 | Protein ID | WP_000520549.1 |
Coordinates | 28530..28778 (-) | Length | 83 a.a. |
Antitoxin (Protein)
Gene name | hicB | Uniprot ID | V0AWG0 |
Locus tag | QQJ99_RS24080 | Protein ID | WP_000681613.1 |
Coordinates | 28186..28533 (-) | Length | 116 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
QQJ99_RS24050 (VEF5_46220) | 24191..24517 | - | 327 | WP_000008647.1 | TrbC/VirB2 family protein | - |
QQJ99_RS24055 (VEF5_46230) | 24531..25151 | - | 621 | WP_159126519.1 | lytic transglycosylase domain-containing protein | - |
QQJ99_RS24060 (VEF5_46250) | 25507..25722 | - | 216 | WP_000760375.1 | EexN family lipoprotein | - |
QQJ99_RS24065 (VEF5_46260) | 25719..26012 | - | 294 | WP_060598542.1 | sigma factor-like helix-turn-helix DNA-binding protein | - |
QQJ99_RS24070 (VEF5_46270) | 26089..27318 | - | 1230 | WP_001329504.1 | DNA relaxase/nickase TaxC | - |
QQJ99_RS24075 (VEF5_46280) | 27322..27825 | - | 504 | WP_000781813.1 | relaxosome-coupling protein TaxA | - |
QQJ99_RS24080 (VEF5_46290) | 28186..28533 | - | 348 | WP_000681613.1 | type II toxin-antitoxin system HicB family antitoxin | Antitoxin |
QQJ99_RS24085 (VEF5_46300) | 28530..28778 | - | 249 | WP_000520549.1 | type II toxin-antitoxin system HicA family toxin | Toxin |
QQJ99_RS24090 (VEF5_46320) | 29083..29433 | - | 351 | WP_000854259.1 | hypothetical protein | - |
QQJ99_RS24095 (VEF5_46330) | 29524..29823 | - | 300 | WP_000650309.1 | hypothetical protein | - |
QQJ99_RS24100 (VEF5_46340) | 30277..30504 | - | 228 | WP_000801440.1 | hypothetical protein | - |
QQJ99_RS24105 (VEF5_46350) | 30491..30742 | - | 252 | WP_001230707.1 | hypothetical protein | - |
QQJ99_RS24110 (VEF5_46360) | 30739..31281 | - | 543 | WP_001083906.1 | hypothetical protein | - |
QQJ99_RS24115 (VEF5_46370) | 31386..31901 | - | 516 | WP_001025395.1 | J domain-containing protein | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | mcr-1.1 | - | 1..33309 | 33309 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 83 a.a. Molecular weight: 9422.08 Da Isoelectric Point: 10.4800
>T44237 WP_000520549.1 NZ_AP027955:c28778-28530 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL
Download Length: 249 bp
>T44237 NZ_AP027955:c28778-28530 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA
Antitoxin
Download Length: 116 a.a. Molecular weight: 13113.84 Da Isoelectric Point: 4.6723
>AT44237 WP_000681613.1 NZ_AP027955:c28533-28186 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA
Download Length: 348 bp
>AT44237 NZ_AP027955:c28533-28186 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A829CIX3 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A829CLC1 |