Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 28186..28778 Replicon plasmid pVE-F5_2
Accession NZ_AP027955
Organism Escherichia coli strain VE-F5

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag QQJ99_RS24085 Protein ID WP_000520549.1
Coordinates 28530..28778 (-) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag QQJ99_RS24080 Protein ID WP_000681613.1
Coordinates 28186..28533 (-) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQJ99_RS24050 (VEF5_46220) 24191..24517 - 327 WP_000008647.1 TrbC/VirB2 family protein -
QQJ99_RS24055 (VEF5_46230) 24531..25151 - 621 WP_159126519.1 lytic transglycosylase domain-containing protein -
QQJ99_RS24060 (VEF5_46250) 25507..25722 - 216 WP_000760375.1 EexN family lipoprotein -
QQJ99_RS24065 (VEF5_46260) 25719..26012 - 294 WP_060598542.1 sigma factor-like helix-turn-helix DNA-binding protein -
QQJ99_RS24070 (VEF5_46270) 26089..27318 - 1230 WP_001329504.1 DNA relaxase/nickase TaxC -
QQJ99_RS24075 (VEF5_46280) 27322..27825 - 504 WP_000781813.1 relaxosome-coupling protein TaxA -
QQJ99_RS24080 (VEF5_46290) 28186..28533 - 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QQJ99_RS24085 (VEF5_46300) 28530..28778 - 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
QQJ99_RS24090 (VEF5_46320) 29083..29433 - 351 WP_000854259.1 hypothetical protein -
QQJ99_RS24095 (VEF5_46330) 29524..29823 - 300 WP_000650309.1 hypothetical protein -
QQJ99_RS24100 (VEF5_46340) 30277..30504 - 228 WP_000801440.1 hypothetical protein -
QQJ99_RS24105 (VEF5_46350) 30491..30742 - 252 WP_001230707.1 hypothetical protein -
QQJ99_RS24110 (VEF5_46360) 30739..31281 - 543 WP_001083906.1 hypothetical protein -
QQJ99_RS24115 (VEF5_46370) 31386..31901 - 516 WP_001025395.1 J domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T44237 WP_000520549.1 NZ_AP027955:c28778-28530 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T44237 NZ_AP027955:c28778-28530 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT44237 WP_000681613.1 NZ_AP027955:c28533-28186 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT44237 NZ_AP027955:c28533-28186 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References