Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4324861..4325358 Replicon chromosome
Accession NZ_AP027953
Organism Escherichia coli strain VE-F5

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QQJ99_RS21230 Protein ID WP_001173343.1
Coordinates 4324861..4325034 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QQJ99_RS21235 Protein ID WP_000398619.1
Coordinates 4325062..4325358 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQJ99_RS21220 (4321531) 4321531..4322988 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
QQJ99_RS21225 (4323225) 4323225..4324742 + 1518 WP_001295074.1 lysine--tRNA ligase -
QQJ99_RS21230 (4324861) 4324861..4325034 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QQJ99_RS21235 (4325062) 4325062..4325358 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QQJ99_RS21240 (4325585) 4325585..4325857 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
QQJ99_RS21245 (4325869) 4325869..4326099 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
QQJ99_RS21250 (4326280) 4326280..4327911 + 1632 WP_001216477.1 sensor histidine kinase -
QQJ99_RS21255 (4327908) 4327908..4328627 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T44236 WP_001173343.1 NZ_AP027953:c4325034-4324861 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T44236 NZ_AP027953:c4325034-4324861 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT44236 WP_000398619.1 NZ_AP027953:c4325358-4325062 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT44236 NZ_AP027953:c4325358-4325062 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References