Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 3668793..3670218 Replicon chromosome
Accession NZ_AP027953
Organism Escherichia coli strain VE-F5

Toxin (Protein)


Gene name rnlA Uniprot ID A0A1X9TDS5
Locus tag QQJ99_RS17960 Protein ID WP_001730411.1
Coordinates 3669148..3670218 (-) Length 357 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID E6BSF0
Locus tag QQJ99_RS17955 Protein ID WP_000461705.1
Coordinates 3668793..3669155 (-) Length 121 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQJ99_RS17935 (3664695) 3664695..3665651 + 957 WP_000121359.1 molybdenum cofactor insertion chaperone PaoD -
QQJ99_RS17940 (3665702) 3665702..3666040 + 339 Protein_3509 LysR substrate-binding domain-containing protein -
QQJ99_RS17945 (3666605) 3666605..3667744 + 1140 WP_000784327.1 hypothetical protein -
QQJ99_RS17955 (3668793) 3668793..3669155 - 363 WP_000461705.1 type II toxin-antitoxin system RnlB family antitoxin Antitoxin
QQJ99_RS17960 (3669148) 3669148..3670218 - 1071 WP_001730411.1 type II toxin-antitoxin system RnlA family toxin Toxin
QQJ99_RS17965 (3670353) 3670353..3670549 - 197 Protein_3514 recombinase family protein -
QQJ99_RS17970 (3670619) 3670619..3671203 - 585 WP_284317960.1 tail fiber assembly protein -
QQJ99_RS17975 (3671203) 3671203..3674481 - 3279 WP_284317961.1 phage tail protein -
QQJ99_RS17980 (3674546) 3674546..3675145 - 600 WP_001233090.1 Ail/Lom family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 3667793..3712737 44944
- flank IS/Tn - - 3667793..3668170 377


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(15-112)


Sequences

Toxin        


Download         Length: 357 a.a.        Molecular weight: 40463.36 Da        Isoelectric Point: 6.8748

>T44231 WP_001730411.1 NZ_AP027953:c3670218-3669148 [Escherichia coli]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANHNL
GKELADDLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGRYFDKIGSEFILKQQFGCNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Download         Length: 1071 bp

>T44231 NZ_AP027953:c3670218-3669148 [Escherichia coli]
ATGACAGTCAGGAACTACACTAATTTAAATTTAGATCGGACAACTATTGAGGCTACGTCTAGGGCATTTATTGAAAATAA
AAACTATTCCGTCCATTCAATTGGACCAATGCCTGGTGCAAGAGCAGGACTTCGGGTTGTGTTTGCTAAACCAGGAGAAG
CTTTGGCAACTGTTAACATTTTCTATAATAACGGAGGGACGTCTACAGTCCAGTATCAAACAGGAGCTAATCATAATCTT
GGAAAAGAGTTGGCTGATGATCTCTATGAAACCATCAATCCTGCAGAATTTGAACAGGTAAATATGGTGTTACAAGGGTT
CGTTGAAGCGAACGTTTTATCTGTACTTCAGCTCTCAGCAGAGCAGCCACATATACAATTTTACGAATACTTACGGAACA
CTCATACAACGGTGTGGAAAATTCATTCGCCAGAATTTCAGGATGAGTTGACAGTTAGTCTGCATCACAGAAATGGAACA
CTACAAATACAAGGGCGTCCATTGTCATGTTATAGAGTGTTTATATTCAACTTGTCAGAGCTACTCGATTTACAAGGACT
GGAAAAAGTTCTCATTCGTCAGGACGATGGCAAAGCGTATATAGTTCAGAAAGAAGTTGCTCGCTCACATCTAGAGTGTG
AAATGGGGGATGCGTACCCACTATTGCATAAGAATGTTGAAAAATTGCTCGTTTCTGGGCTGTGTGTTAAACTCGCCGCC
CCCGATTTACCCGATTACTGTATGTTGTTGTATCCTGAATTACGTTCAGTTGAGGGAGTACTTAAATCAACAATGTACCG
ATATGGCATGCCAATCACGCAGGATGGTTTCGGGCGTTATTTTGATAAAATTGGGAGTGAATTTATTCTGAAACAACAAT
TTGGATGCAATTTATCGTCAGCTAAGGTTAAGACCATTAATGATGCCTATACTTTCTTTAACAGAGAACGTCATGGCCTG
TTTCATATGGAGATAGTTGTCGACACAAGCCGTATGGTTTCTGACATGGCACGACTGATGACTAAGGCCAGACAGGCATG
GGGAATTATTAAGGATTTGTATATTGTTTGA

Antitoxin


Download         Length: 121 a.a.        Molecular weight: 13304.23 Da        Isoelectric Point: 4.2386

>AT44231 WP_000461705.1 NZ_AP027953:c3669155-3668793 [Escherichia coli]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Download         Length: 363 bp

>AT44231 NZ_AP027953:c3669155-3668793 [Escherichia coli]
TTGTTTGAAATAACAGGTGTTAACGTCTCAGGGGCTCTCATTGCCGTCGTTATGGCGACTGGCTACGAGAACCCCTTGAC
TTCAGTTCACGAAATCGAGAAGAGGCTTTCTGTTATACTGGGCAATTCACAGGCTACAGGTGAGGTTTTGTTTGACCTTT
TGTGTTCTAATGGTCCAGAGTGGAATCGCTTTGTTTCGCTGGAAATAAGGTGTGGCAGTATTTTGCTGGATACTGCCAAA
ATTGTGGATGAGCAAGAAATTCCAGCGCAGATCTTGAGACATCAGAGAGATGTATTACGTAACCACCCTGAGTACGTAGA
AGACTCTGTGTTAACACCAGATGATGTTCTTTGTATTCTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1X9TDS5

Antitoxin

Source ID Structure
AlphaFold DB A0A1X9TDS3

References