Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3483019..3483637 Replicon chromosome
Accession NZ_AP027953
Organism Escherichia coli strain VE-F5

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QQJ99_RS17070 Protein ID WP_001291435.1
Coordinates 3483419..3483637 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QQJ99_RS17065 Protein ID WP_000344800.1
Coordinates 3483019..3483393 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQJ99_RS17055 (3478108) 3478108..3479301 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QQJ99_RS17060 (3479324) 3479324..3482473 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QQJ99_RS17065 (3483019) 3483019..3483393 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QQJ99_RS17070 (3483419) 3483419..3483637 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QQJ99_RS17075 (3483809) 3483809..3484360 + 552 WP_000102564.1 maltose O-acetyltransferase -
QQJ99_RS17080 (3484476) 3484476..3484946 + 471 WP_000136192.1 YlaC family protein -
QQJ99_RS17085 (3485110) 3485110..3486660 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QQJ99_RS17090 (3486702) 3486702..3487055 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QQJ99_RS17100 (3487434) 3487434..3487745 + 312 WP_000409911.1 MGMT family protein -
QQJ99_RS17105 (3487776) 3487776..3488348 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44230 WP_001291435.1 NZ_AP027953:3483419-3483637 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44230 NZ_AP027953:3483419-3483637 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44230 WP_000344800.1 NZ_AP027953:3483019-3483393 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44230 NZ_AP027953:3483019-3483393 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References