Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) hicAB/-
Location 10725..11317 Replicon plasmid pVE-F10_2
Accession NZ_AP027948
Organism Escherichia coli strain VE-F10

Toxin (Protein)


Gene name hicA Uniprot ID A0A1I9ZJC5
Locus tag QQK65_RS23935 Protein ID WP_000513532.1
Coordinates 10725..10973 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A1I9ZJ49
Locus tag QQK65_RS23940 Protein ID WP_000681609.1
Coordinates 10970..11317 (+) Length 116 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK65_RS23900 (VEF10_45810) 5729..6394 - 666 WP_000532148.1 division plane positioning ATPase MipZ -
QQK65_RS23905 (VEF10_45820) 6983..7312 + 330 WP_000855119.1 hypothetical protein -
QQK65_RS23910 (VEF10_45830) 7716..8231 + 516 WP_001025393.1 J domain-containing protein -
QQK65_RS23915 (VEF10_45840) 8335..8877 + 543 WP_001083904.1 hypothetical protein -
QQK65_RS23920 (VEF10_45850) 8874..9128 + 255 WP_001230709.1 hypothetical protein -
QQK65_RS23925 (VEF10_45860) 9125..9367 + 243 WP_000151456.1 hypothetical protein -
QQK65_RS23930 (VEF10_45870) 9938..10231 + 294 WP_000673428.1 hypothetical protein -
QQK65_RS23935 (VEF10_45890) 10725..10973 + 249 WP_000513532.1 type II toxin-antitoxin system HicA family toxin Toxin
QQK65_RS23940 (VEF10_45900) 10970..11317 + 348 WP_000681609.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QQK65_RS23945 (VEF10_45910) 11405..11848 - 444 WP_023223351.1 hypothetical protein -
QQK65_RS23950 (VEF10_45920) 12089..12388 + 300 WP_034169410.1 type II toxin-antitoxin system HicB family antitoxin -
QQK65_RS23955 (VEF10_45930) 12648..12974 - 327 WP_000206697.1 hypothetical protein -
QQK65_RS23960 (VEF10_45940) 12976..13299 - 324 WP_223666488.1 hypothetical protein -
QQK65_RS23965 13276..13416 - 141 Protein_21 hypothetical protein -
QQK65_RS23970 (VEF10_45950) 13436..14143 - 708 WP_063084541.1 hypothetical protein -
QQK65_RS23975 (VEF10_45960) 14145..14897 - 753 WP_000797967.1 conjugal transfer protein TraL -
QQK65_RS23980 (VEF10_45970) 14915..15316 - 402 WP_063084542.1 hypothetical protein -
QQK65_RS23985 (VEF10_45980) 15684..16025 + 342 WP_000730722.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mcr-1.1 - 1..63710 63710


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(20-81)

Antitoxin

(54-104)


Sequences


Toxin        


Download         Length: 83 a.a.        Molecular weight: 9378.03 Da        Isoelectric Point: 10.4800

>T44209 WP_000513532.1 NZ_AP027948:10725-10973 [Escherichia coli]
MGKADKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
VL

Download         Length: 249 bp

>T44209 NZ_AP027948:10725-10973 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGCAAAATTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTCGTTGTTTTGTT
TTCTTCTCTAGGATATGTCAAAAAGGAAATGCAGGGATCAAGAGTGCGATTTTTCAATGCTGAAATTAATCACACAATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCTATTAAACAGAACTTGAAAGAGGCTGGG
GTTTTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13206.97 Da        Isoelectric Point: 4.8313

>AT44209 WP_000681609.1 NZ_AP027948:10970-11317 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNILYGKLAFIRDLVTYEAETLADLEREFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSHHRAGA

Download         Length: 348 bp

>AT44209 NZ_AP027948:10970-11317 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGTACGGTTGAGCCGGATTTTGAAAATAATATCCTGTATGGAAAGCTGGC
ATTTATTCGTGATCTGGTGACTTATGAGGCAGAAACATTGGCTGACCTGGAACGGGAATTTAAAACATCGGTTGATTTGT
ATTTACAGTCCTGTGTGGAGGACGGAAAGGAGCCTGACACGCCCTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAG
CTGCATCGTCGGGTCGCTGAAATGGCGATGGAAGAAGATTTGTCGCTGAATGCCTTTGTCAATAAGGCACTGGAAAAAGA
AGTCAGTCATCATCGCGCAGGGGCTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A1I9ZJC5


Antitoxin

Source ID Structure
AlphaFold DB A0A1I9ZJ49

References