Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | TsxAB/MqsA(antitoxin) |
| Location | 8874..9367 | Replicon | plasmid pVE-F10_2 |
| Accession | NZ_AP027948 | ||
| Organism | Escherichia coli strain VE-F10 | ||
Toxin (Protein)
| Gene name | TsxA | Uniprot ID | U9Y9W8 |
| Locus tag | QQK65_RS23920 | Protein ID | WP_001230709.1 |
| Coordinates | 8874..9128 (+) | Length | 85 a.a. |
Antitoxin (Protein)
| Gene name | TsxB | Uniprot ID | U9Y4R8 |
| Locus tag | QQK65_RS23925 | Protein ID | WP_000151456.1 |
| Coordinates | 9125..9367 (+) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QQK65_RS23880 (VEF10_45780) | 4388..4540 | + | 153 | Protein_4 | DUF5431 family protein | - |
| QQK65_RS23885 | 4485..4607 | + | 123 | WP_223170659.1 | type I toxin-antitoxin system Hok family toxin | - |
| QQK65_RS23890 (VEF10_45790) | 4757..5353 | + | 597 | WP_000125165.1 | ProQ/FINO family protein | - |
| QQK65_RS23895 (VEF10_45800) | 5371..5655 | - | 285 | WP_001162371.1 | hypothetical protein | - |
| QQK65_RS23900 (VEF10_45810) | 5729..6394 | - | 666 | WP_000532148.1 | division plane positioning ATPase MipZ | - |
| QQK65_RS23905 (VEF10_45820) | 6983..7312 | + | 330 | WP_000855119.1 | hypothetical protein | - |
| QQK65_RS23910 (VEF10_45830) | 7716..8231 | + | 516 | WP_001025393.1 | J domain-containing protein | - |
| QQK65_RS23915 (VEF10_45840) | 8335..8877 | + | 543 | WP_001083904.1 | hypothetical protein | - |
| QQK65_RS23920 (VEF10_45850) | 8874..9128 | + | 255 | WP_001230709.1 | hypothetical protein | Toxin |
| QQK65_RS23925 (VEF10_45860) | 9125..9367 | + | 243 | WP_000151456.1 | hypothetical protein | Antitoxin |
| QQK65_RS23930 (VEF10_45870) | 9938..10231 | + | 294 | WP_000673428.1 | hypothetical protein | - |
| QQK65_RS23935 (VEF10_45890) | 10725..10973 | + | 249 | WP_000513532.1 | type II toxin-antitoxin system HicA family toxin | - |
| QQK65_RS23940 (VEF10_45900) | 10970..11317 | + | 348 | WP_000681609.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| QQK65_RS23945 (VEF10_45910) | 11405..11848 | - | 444 | WP_023223351.1 | hypothetical protein | - |
| QQK65_RS23950 (VEF10_45920) | 12089..12388 | + | 300 | WP_034169410.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| QQK65_RS23955 (VEF10_45930) | 12648..12974 | - | 327 | WP_000206697.1 | hypothetical protein | - |
| QQK65_RS23960 (VEF10_45940) | 12976..13299 | - | 324 | WP_223666488.1 | hypothetical protein | - |
| QQK65_RS23965 | 13276..13416 | - | 141 | Protein_21 | hypothetical protein | - |
| QQK65_RS23970 (VEF10_45950) | 13436..14143 | - | 708 | WP_063084541.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | mcr-1.1 | - | 1..63710 | 63710 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 85 a.a. Molecular weight: 9942.47 Da Isoelectric Point: 10.1206
>T44208 WP_001230709.1 NZ_AP027948:8874-9128 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA
Download Length: 255 bp
>T44208 NZ_AP027948:8874-9128 [Escherichia coli]
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA
ATGAGAAAAAACAAAGGGCGATTGACTTATTATCTTGAAGTGATTGATAAAAAATACCATTTTGTAAAAAAAATAAGCAG
TTATTCAAAGGAGTTCACTGACGGAAAAACAAAAAGAACAAAGAGAACGTTAAGTGAGCTGGTTTTTAATGAAAGTGAGG
TCGAGGCAATAGACTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGGTGAAAGAATATAAG
GAGAGTGATGCATGA
Antitoxin
Download Length: 81 a.a. Molecular weight: 9136.45 Da Isoelectric Point: 7.2775
>AT44208 WP_000151456.1 NZ_AP027948:9125-9367 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP
Download Length: 243 bp
>AT44208 NZ_AP027948:9125-9367 [Escherichia coli]
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA
ATGACTATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATTGGCCTTACCCAGACAGAGTGCGGTGAGAT
TTTTGGCGTAGGCTTAAGTACATGGCAGAAAAAAGAAGCTAAAACCCACAACCAGCTTAATTTATCGAAAGGTGAATTTG
AGTACCTGTTATTACTTGCAGGGGAGCACCCTGAATACGTCCTGCATAAAAAAGCTGAATCCCGCTCACCGGAGGAACCG
TGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9H8F0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3G9GNF5 |