Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4032818..4033394 Replicon chromosome
Accession NZ_AP027946
Organism Escherichia coli strain VE-F10

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QQK65_RS19485 Protein ID WP_001297643.1
Coordinates 4033107..4033394 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QQK65_RS19480 Protein ID WP_000063156.1
Coordinates 4032818..4033120 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK65_RS19465 (4029414) 4029414..4031564 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QQK65_RS19470 (4031614) 4031614..4031817 + 204 WP_000467859.1 YbdD/YjiX family protein -
QQK65_RS19475 (4031828) 4031828..4032784 + 957 WP_001297640.1 GTPase -
QQK65_RS19480 (4032818) 4032818..4033120 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QQK65_RS19485 (4033107) 4033107..4033394 - 288 WP_001297643.1 BrnT family toxin Toxin
QQK65_RS19490 (4033593) 4033593..4034290 - 698 Protein_3807 IS1 family transposase -
QQK65_RS19495 (4034347) 4034347..4034442 + 96 Protein_3808 dienelactone hydrolase family protein -
QQK65_RS19500 (4034588) 4034588..4035820 + 1233 WP_001339512.1 multidrug efflux MFS transporter MdtM -
QQK65_RS19505 (4035861) 4035861..4037141 + 1281 WP_001037380.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T44205 WP_001297643.1 NZ_AP027946:c4033394-4033107 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T44205 NZ_AP027946:c4033394-4033107 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT44205 WP_000063156.1 NZ_AP027946:c4033120-4032818 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT44205 NZ_AP027946:c4033120-4032818 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References