Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3517713..3518331 Replicon chromosome
Accession NZ_AP027946
Organism Escherichia coli strain VE-F10

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QQK65_RS17030 Protein ID WP_001291435.1
Coordinates 3518113..3518331 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QQK65_RS17025 Protein ID WP_000344800.1
Coordinates 3517713..3518087 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK65_RS17015 (3512802) 3512802..3513995 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QQK65_RS17020 (3514018) 3514018..3517167 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QQK65_RS17025 (3517713) 3517713..3518087 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QQK65_RS17030 (3518113) 3518113..3518331 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QQK65_RS17035 (3518503) 3518503..3519054 + 552 WP_000102564.1 maltose O-acetyltransferase -
QQK65_RS17040 (3519170) 3519170..3519640 + 471 WP_000136192.1 YlaC family protein -
QQK65_RS17045 (3519922) 3519922..3520619 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
QQK65_RS17050 (3520629) 3520629..3522131 + 1503 WP_284344114.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QQK65_RS17055 (3522173) 3522173..3522526 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QQK65_RS17065 (3522905) 3522905..3523216 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44202 WP_001291435.1 NZ_AP027946:3518113-3518331 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44202 NZ_AP027946:3518113-3518331 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44202 WP_000344800.1 NZ_AP027946:3517713-3518087 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44202 NZ_AP027946:3517713-3518087 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References