Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2460872..2461510 Replicon chromosome
Accession NZ_AP027946
Organism Escherichia coli strain VE-F10

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QQK65_RS11905 Protein ID WP_000813794.1
Coordinates 2461334..2461510 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QQK65_RS11900 Protein ID WP_001270286.1
Coordinates 2460872..2461288 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK65_RS11880 (2456024) 2456024..2456965 - 942 WP_063123347.1 ABC transporter permease -
QQK65_RS11885 (2456966) 2456966..2457979 - 1014 WP_000220396.1 ABC transporter ATP-binding protein -
QQK65_RS11890 (2457997) 2457997..2459142 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
QQK65_RS11895 (2459387) 2459387..2460793 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
QQK65_RS11900 (2460872) 2460872..2461288 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QQK65_RS11905 (2461334) 2461334..2461510 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QQK65_RS11910 (2461732) 2461732..2461962 + 231 WP_000494244.1 YncJ family protein -
QQK65_RS11915 (2462054) 2462054..2464015 - 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QQK65_RS11920 (2464088) 2464088..2464624 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QQK65_RS11925 (2464677) 2464677..2465891 + 1215 WP_001326689.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T44200 WP_000813794.1 NZ_AP027946:c2461510-2461334 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T44200 NZ_AP027946:c2461510-2461334 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT44200 WP_001270286.1 NZ_AP027946:c2461288-2460872 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT44200 NZ_AP027946:c2461288-2460872 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References