Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 867598..868252 Replicon chromosome
Accession NZ_AP027946
Organism Escherichia coli strain VE-F10

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QQK65_RS04305 Protein ID WP_000244777.1
Coordinates 867845..868252 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QQK65_RS04300 Protein ID WP_000354046.1
Coordinates 867598..867864 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QQK65_RS04275 (862767) 862767..863510 + 744 WP_000951964.1 SDR family oxidoreductase -
QQK65_RS04280 (863567) 863567..865000 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
QQK65_RS04285 (865045) 865045..865356 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QQK65_RS04290 (865520) 865520..866179 + 660 WP_000250274.1 hemolysin III family protein -
QQK65_RS04295 (866375) 866375..867355 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QQK65_RS04300 (867598) 867598..867864 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QQK65_RS04305 (867845) 867845..868252 + 408 WP_000244777.1 protein YgfX Toxin
QQK65_RS04310 (868292) 868292..868813 - 522 WP_001055874.1 flavodoxin FldB -
QQK65_RS04315 (868925) 868925..869821 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QQK65_RS04320 (869846) 869846..870556 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QQK65_RS04325 (870562) 870562..872295 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T44188 WP_000244777.1 NZ_AP027946:867845-868252 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T44188 NZ_AP027946:867845-868252 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT44188 WP_000354046.1 NZ_AP027946:867598-867864 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT44188 NZ_AP027946:867598-867864 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References