Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1140821..1141475 Replicon chromosome
Accession NZ_AP027926
Organism Escherichia fergusonii strain 30038

Toxin (Protein)


Gene name cptA Uniprot ID F0JPE9
Locus tag QUE84_RS05435 Protein ID WP_000244774.1
Coordinates 1141068..1141475 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE84_RS05430 Protein ID WP_000354046.1
Coordinates 1140821..1141087 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE84_RS05405 (1135959) 1135959..1136852 + 894 WP_000819362.1 transporter -
QUE84_RS05410 (1136900) 1136900..1138333 - 1434 WP_024256637.1 6-phospho-beta-glucosidase BglA -
QUE84_RS05415 (1138378) 1138378..1138689 + 312 WP_001182961.1 N(4)-acetylcytidine aminohydrolase -
QUE84_RS05420 (1138853) 1138853..1139512 + 660 WP_223689698.1 hemolysin III family protein -
QUE84_RS05425 (1139589) 1139589..1140569 - 981 WP_032171975.1 tRNA-modifying protein YgfZ -
QUE84_RS05430 (1140821) 1140821..1141087 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE84_RS05435 (1141068) 1141068..1141475 + 408 WP_000244774.1 protein YgfX Toxin
QUE84_RS05440 (1141515) 1141515..1142036 - 522 WP_001055874.1 flavodoxin FldB -
QUE84_RS05445 (1142148) 1142148..1143044 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE84_RS05450 (1143069) 1143069..1143779 + 711 WP_000715223.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE84_RS05455 (1143785) 1143785..1145518 + 1734 WP_223684717.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16078.05 Da        Isoelectric Point: 11.2511

>T44158 WP_000244774.1 NZ_AP027926:1141068-1141475 [Escherichia fergusonii]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T44158 NZ_AP027926:1141068-1141475 [Escherichia fergusonii]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGTTAAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCCCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCGCCGTGGATGATTAAAAGCGGCATGATGTTGCGTTTACGTTCTGATAGCGGTAAACGTCA
GCACTTGTGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT44158 WP_000354046.1 NZ_AP027926:1140821-1141087 [Escherichia fergusonii]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT44158 NZ_AP027926:1140821-1141087 [Escherichia fergusonii]
ATGGACATTAATAACAAAGCCCGCATTCACTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGTGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTCAACTGGCTGATGAATCATGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L6ZX35

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References