Detailed information of TA system TA044141

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Fic(toxin)
• Location: 49608..50176
• Replicon: plasmid pNBRC108728a
• Accession: NZ_AP027733
• Organism: Frondihabitans sucicola strain NBRC 108728

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: QUE79_RS22140
• Protein ID: WP_286347420.1
• Coordinates: 49829..50176 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: QUE79_RS22135
• Protein ID: WP_286347225.1
• Coordinates: 49608..49799 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE79_RS22105 (GCM10025867_46010)	45120..45620	-	501	WP_286347219.1	hypothetical protein	-
QUE79_RS22110 (GCM10025867_46020)	45717..46301	-	585	WP_286347220.1	hypothetical protein	-
QUE79_RS22115 (GCM10025867_46030)	46482..46790	-	309	WP_286347221.1	hypothetical protein	-
QUE79_RS22120 (GCM10025867_46040)	46855..48489	-	1635	WP_286347222.1	hypothetical protein	-
QUE79_RS22125 (GCM10025867_46050)	48564..48971	+	408	WP_286347223.1	hypothetical protein	-
QUE79_RS22130 (GCM10025867_46060)	49191..49376	-	186	WP_286347224.1	hypothetical protein	-
QUE79_RS22135 (GCM10025867_46070)	49608..49799	+	192	WP_286347225.1	antitoxin Phd	Antitoxin
QUE79_RS22140 (GCM10025867_46080)	49829..50176	+	348	WP_286347420.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
QUE79_RS22145 (GCM10025867_46090)	50285..50827	-	543	WP_286347226.1	hypothetical protein	-
QUE79_RS22150 (GCM10025867_46100)	50824..51111	-	288	WP_286347227.1	hypothetical protein	-
QUE79_RS22155 (GCM10025867_46110)	51108..51485	-	378	WP_286347228.1	hypothetical protein	-
QUE79_RS22160 (GCM10025867_46120)	51603..52421	-	819	WP_286347229.1	hypothetical protein	-
QUE79_RS22165 (GCM10025867_46130)	52460..52606	-	147	WP_286347230.1	hypothetical protein	-
QUE79_RS22170 (GCM10025867_46140)	52932..53663	-	732	WP_286347231.1	hypothetical protein	-
QUE79_RS22175 (GCM10025867_46150)	53660..54070	-	411	WP_286347232.1	hypothetical protein	-
QUE79_RS22180 (GCM10025867_46160)	54063..54758	-	696	WP_286347233.1	hypothetical protein	-
QUE79_RS22185 (GCM10025867_46170)	54755..55024	-	270	WP_286347234.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..449692	449692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (30-107)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12135.67 Da
• Isoelectric Point: 4.1122

>T44141 WP_286347420.1 NZ_AP027733:49829-50176 [Frondihabitans sucicola]
MTAGSIAYGQPVAVRDFGLLDAAVARPQSVVFGYDAYPDLWQKAAALLHSVARNHSLVDGNKRTAWAAAWVFLGCNGVTL
AAEFDVDAAYDFMIAAATGDLDVPEIATALEAFAE

Nucleotide

• Length: 348 bp

>T44141 NZ_AP027733:49829-50176 [Frondihabitans sucicola]
ATGACCGCAGGCTCCATCGCCTACGGACAACCCGTCGCGGTCCGCGACTTCGGCCTCCTCGATGCCGCCGTCGCGCGCCC
GCAAAGCGTCGTGTTCGGCTACGACGCCTACCCGGACCTCTGGCAGAAGGCGGCAGCCCTCCTGCATTCCGTCGCGCGGA
ACCATTCCCTCGTGGACGGCAACAAGCGCACGGCATGGGCGGCCGCGTGGGTGTTCCTCGGGTGCAATGGCGTCACCCTC
GCCGCCGAATTCGACGTGGATGCCGCCTATGACTTCATGATCGCGGCCGCCACCGGTGACCTCGACGTTCCGGAGATCGC
GACAGCCCTTGAGGCGTTCGCGGAGTAG

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 6942.82 Da
• Isoelectric Point: 5.7551

>AT44141 WP_286347225.1 NZ_AP027733:49608-49799 [Frondihabitans sucicola]
MPSLNVTFTDDELEEIRQAATDTSLKTYVHDSALNAARTRKALVAQLARQVAEKSAVLNERLA

Nucleotide

• Length: 192 bp

>AT44141 NZ_AP027733:49608-49799 [Frondihabitans sucicola]
ATGCCCTCACTGAATGTGACCTTCACCGACGACGAACTCGAAGAGATTCGCCAGGCCGCCACGGACACCTCGCTCAAGAC
GTACGTGCACGACAGCGCCCTCAACGCCGCCCGCACCCGCAAAGCCCTCGTCGCCCAGCTCGCACGGCAGGTCGCCGAGA
AGTCCGCTGTGCTCAACGAGCGCCTCGCGTGA