Detailed information of TA system TA044129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 867440..868072
• Replicon: chromosome
• Accession: NZ_AP027452
• Organism: Mycolicibacterium mageritense strain H4_3_1

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: QUE81_RS04205
• Protein ID: WP_229480287.1
• Coordinates: 867440..867889 (-)
• Length: 150 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X5LDU2
• Locus tag: QUE81_RS04210
• Protein ID: WP_036433724.1
• Coordinates: 867893..868072 (-)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE81_RS04180 (hbim_00841)	863847..864101	+	255	WP_229480292.1	hypothetical protein	-
QUE81_RS04185 (hbim_00842)	864111..865853	+	1743	WP_276821621.1	fumarate reductase (quinol) flavoprotein subunit	-
QUE81_RS04190 (hbim_00843)	865855..866595	+	741	WP_286213626.1	succinate dehydrogenase/fumarate reductase iron-sulfur subunit	-
QUE81_RS04195 (hbim_00844)	866592..866966	+	375	WP_286213627.1	fumarate reductase subunit C	-
QUE81_RS04200 (hbim_00845)	866963..867322	+	360	WP_230021378.1	fumarate reductase subunit FrdD	-
QUE81_RS04205 (hbim_00846)	867440..867889	-	450	WP_229480287.1	SRPBCC family protein	Toxin
QUE81_RS04210 (hbim_00847)	867893..868072	-	180	WP_036433724.1	antitoxin	Antitoxin
QUE81_RS04215 (hbim_00848)	868143..870629	-	2487	WP_286213629.1	HAD-IC family P-type ATPase	-
QUE81_RS04220 (hbim_00849)	870658..872190	-	1533	WP_286216450.1	serine hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-145)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 150 a.a.
• Molecular weight: 16139.72 Da
• Isoelectric Point: 9.6575

>T44129 WP_229480287.1 NZ_AP027452:c867889-867440 [Mycolicibacterium mageritense]
MAKLSVSVDVPLPPEKAWEYASDLSRYDEWLSIHRAWRSKLPETLEKGTVIDSIVEVKGMLNRVKWTLVNYKPPHSLTLN
GDGRGGVKVKLIGKIKPAPVDGAEGATVTFDVHLGGPALFGPIGMVVASALKGDIQESLNKFKALYASS

Nucleotide

• Length: 450 bp

>T44129 NZ_AP027452:c867889-867440 [Mycolicibacterium mageritense]
ATGGCAAAACTGTCAGTCTCCGTCGATGTGCCGTTGCCGCCGGAGAAGGCGTGGGAGTACGCCTCCGATCTGTCCCGTTA
CGACGAGTGGTTGAGCATCCACCGCGCCTGGCGGTCGAAGTTGCCGGAAACCCTTGAGAAGGGCACCGTCATCGACTCGA
TCGTCGAGGTCAAGGGCATGCTCAACCGGGTCAAGTGGACCCTCGTCAACTACAAGCCGCCGCATTCGTTGACCCTCAAC
GGCGACGGCCGCGGCGGGGTCAAAGTCAAGCTGATCGGAAAGATCAAGCCCGCGCCGGTCGACGGCGCCGAGGGCGCCAC
CGTGACGTTCGACGTCCATCTCGGCGGCCCGGCGCTGTTCGGTCCGATCGGCATGGTGGTGGCGTCCGCGCTCAAAGGCG
ACATCCAGGAGTCGCTCAACAAGTTCAAGGCGCTCTACGCATCGTCGTAG

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6443.21 Da
• Isoelectric Point: 8.5694

>AT44129 WP_036433724.1 NZ_AP027452:c868072-867893 [Mycolicibacterium mageritense]
MGFLDKAKDLLSKNADKVDTAIDKAGDVLDQKTQGKYSQHVDKAQDAAKNAIRKEGPQQ

Nucleotide

• Length: 180 bp

>AT44129 NZ_AP027452:c868072-867893 [Mycolicibacterium mageritense]
ATGGGATTTTTGGACAAGGCGAAAGATCTGCTTTCCAAGAACGCCGACAAGGTGGACACGGCGATCGACAAGGCCGGTGA
CGTCCTCGATCAGAAGACGCAGGGCAAGTACAGCCAGCATGTCGACAAGGCGCAGGATGCCGCCAAGAACGCCATCCGTA
AAGAAGGGCCCCAGCAGTAA

Structures

Antitoxin

• AlphaFold DB: X5LDU2