Detailed information of TA system TA044127

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yeeU/CbtA-CbeA
• Location: 3945475..3946196
• Replicon: chromosome
• Accession: NZ_AP027445
• Organism: Escherichia coli strain EC291 isolate EC291

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: QUE55_RS21155
• Protein ID: WP_047399350.1
• Coordinates: 3945475..3945816 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: QUE55_RS21160
• Protein ID: WP_032676674.1
• Coordinates: 3945852..3946196 (-)
• Length: 115 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE55_RS21125 (3941412)	3941412..3942062	-	651	WP_001037966.1	HNH endonuclease	-
QUE55_RS21130 (3942199)	3942199..3942342	+	144	Protein_3824	HNH endonuclease	-
QUE55_RS21135 (3943006)	3943006..3943803	-	798	WP_071701444.1	helix-turn-helix transcriptional regulator	-
QUE55_RS21140 (3943923)	3943923..3944198	-	276	WP_001683517.1	type II toxin-antitoxin system RelE/ParE family toxin	-
QUE55_RS21145 (3944202)	3944202..3944450	-	249	WP_000535212.1	ribbon-helix-helix domain-containing protein	-
QUE55_RS21150 (3944527)	3944527..3945360	-	834	WP_071701443.1	DUF4942 domain-containing protein	-
QUE55_RS21155 (3945475)	3945475..3945816	-	342	WP_047399350.1	TA system toxin CbtA family protein	Toxin
QUE55_RS21160 (3945852)	3945852..3946196	-	345	WP_032676674.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QUE55_RS21165 (3946220)	3946220..3946441	-	222	WP_045265422.1	DUF987 family protein	-
QUE55_RS21170 (3946452)	3946452..3946925	-	474	WP_025376489.1	DNA repair protein RadC	-
QUE55_RS21175 (3946985)	3946985..3947806	-	822	WP_071701442.1	DUF932 domain-containing protein	-
QUE55_RS21180 (3947875)	3947875..3948330	-	456	WP_047399359.1	hypothetical protein	-
QUE55_RS21185 (3948562)	3948562..3949365	-	804	WP_071701440.1	hypothetical protein	-
QUE55_RS21190 (3949385)	3949385..3949819	-	435	WP_149506380.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimB	3936021..3968893	32872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-108)

Antitoxin

• Antitoxin CbeA (14-113)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12748.67 Da
• Isoelectric Point: 8.5196

>T44127 WP_047399350.1 NZ_AP027445:c3945816-3945475 [Escherichia coli]
MNTSPATNQRVAKPCPSPVAVWQMLLTRLLAQHYGLALSDTPFCDETVIQEHINAGVTLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAVDILRARQAMGLLRQGHNLSTR

Nucleotide

• Length: 342 bp

>T44127 NZ_AP027445:c3945816-3945475 [Escherichia coli]
ATGAACACCTCTCCTGCAACAAATCAGCGGGTAGCGAAGCCATGCCCGTCGCCTGTGGCCGTCTGGCAGATGTTACTGAC
CCGTCTACTTGCACAACACTATGGCCTGGCCCTGAGTGACACGCCATTCTGCGATGAAACAGTCATTCAGGAACATATCA
ACGCAGGCGTTACTCTTGCCGATGCCGTGAACTTTCTGGTGGAAAAATACGAACTCGTTCGTATCGATCGCAAAGGATTT
AGCTGGCAGGAACAATCGCCTTATCTGCGGGCTGTAGACATTTTGCGGGCGCGACAGGCAATGGGGTTGTTACGACAAGG
CCATAATCTTTCGACACGCTGA

Antitoxin

Protein

• Length: 115 a.a.
• Molecular weight: 12877.92 Da
• Isoelectric Point: 8.1459

>AT44127 WP_032676674.1 NZ_AP027445:c3946196-3945852 [Escherichia coli]
MKKDTANEVVDTLRWGLKCDITPHFGARLVQEGNRLHYLADRASIIGPFSKADLRHLNQAFPLLMKQMELMLTSGELNPR
HQHCVTLYAKGLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 345 bp

>AT44127 NZ_AP027445:c3946196-3945852 [Escherichia coli]
ATGAAAAAAGACACAGCCAATGAAGTAGTGGATACGCTGCGATGGGGACTGAAATGCGACATCACACCACATTTCGGGGC
CCGTCTGGTGCAGGAAGGCAACCGCCTGCATTACCTCGCCGATCGGGCCAGCATAATCGGTCCGTTCAGCAAAGCTGATT
TACGCCATCTCAACCAGGCTTTCCCCTTGTTGATGAAGCAAATGGAGCTCATGCTCACCAGCGGAGAACTTAATCCCCGG
CACCAGCACTGCGTCACGCTGTATGCGAAAGGATTAACCTGTGAAGCCGATACGCTCGGCTCTTGTGGCTACGTCTATAT
CGCCATTTACCCCACTCAACGCTGA