Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3906243..3906819 Replicon chromosome
Accession NZ_AP027445
Organism Escherichia coli strain EC291 isolate EC291

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QUE55_RS20930 Protein ID WP_001297643.1
Coordinates 3906532..3906819 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QUE55_RS20925 Protein ID WP_000063156.1
Coordinates 3906243..3906545 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE55_RS20910 (3902839) 3902839..3904989 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
QUE55_RS20915 (3905039) 3905039..3905242 + 204 WP_000467859.1 YbdD/YjiX family protein -
QUE55_RS20920 (3905253) 3905253..3906209 + 957 WP_001297640.1 GTPase -
QUE55_RS20925 (3906243) 3906243..3906545 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QUE55_RS20930 (3906532) 3906532..3906819 - 288 WP_001297643.1 BrnT family toxin Toxin
QUE55_RS20935 (3907018) 3907018..3907715 - 698 Protein_3785 IS1 family transposase -
QUE55_RS20940 (3907772) 3907772..3907867 + 96 Protein_3786 dienelactone hydrolase family protein -
QUE55_RS20945 (3908013) 3908013..3909245 + 1233 WP_024186283.1 multidrug efflux MFS transporter MdtM -
QUE55_RS20950 (3909286) 3909286..3910566 + 1281 WP_001037400.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T44126 WP_001297643.1 NZ_AP027445:c3906819-3906532 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T44126 NZ_AP027445:c3906819-3906532 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGTATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT44126 WP_000063156.1 NZ_AP027445:c3906545-3906243 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT44126 NZ_AP027445:c3906545-3906243 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References