Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3424048..3424666 Replicon chromosome
Accession NZ_AP027445
Organism Escherichia coli strain EC291 isolate EC291

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE55_RS18605 Protein ID WP_001291435.1
Coordinates 3424448..3424666 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE55_RS18600 Protein ID WP_000344800.1
Coordinates 3424048..3424422 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE55_RS18590 (3419137) 3419137..3420330 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QUE55_RS18595 (3420353) 3420353..3423502 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QUE55_RS18600 (3424048) 3424048..3424422 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE55_RS18605 (3424448) 3424448..3424666 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE55_RS18610 (3424838) 3424838..3425389 + 552 WP_000102564.1 maltose O-acetyltransferase -
QUE55_RS18615 (3425505) 3425505..3425975 + 471 WP_000136192.1 YlaC family protein -
QUE55_RS18620 (3426139) 3426139..3427689 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE55_RS18625 (3427731) 3427731..3428084 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE55_RS18635 (3428463) 3428463..3428774 + 312 WP_000409911.1 MGMT family protein -
QUE55_RS18640 (3428805) 3428805..3429377 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T44123 WP_001291435.1 NZ_AP027445:3424448-3424666 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T44123 NZ_AP027445:3424448-3424666 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT44123 WP_000344800.1 NZ_AP027445:3424048-3424422 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT44123 NZ_AP027445:3424048-3424422 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References