Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 884886..885540 Replicon chromosome
Accession NZ_AP027445
Organism Escherichia coli strain EC291 isolate EC291

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QUE55_RS05975 Protein ID WP_000244777.1
Coordinates 885133..885540 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE55_RS05970 Protein ID WP_000354046.1
Coordinates 884886..885152 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE55_RS05945 (880055) 880055..880798 + 744 WP_000951948.1 SDR family oxidoreductase -
QUE55_RS05950 (880855) 880855..882288 - 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
QUE55_RS05955 (882333) 882333..882644 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
QUE55_RS05960 (882808) 882808..883467 + 660 WP_000250274.1 hemolysin III family protein -
QUE55_RS05965 (883663) 883663..884643 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUE55_RS05970 (884886) 884886..885152 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE55_RS05975 (885133) 885133..885540 + 408 WP_000244777.1 protein YgfX Toxin
QUE55_RS05980 (885580) 885580..886101 - 522 WP_001055874.1 flavodoxin FldB -
QUE55_RS05985 (886213) 886213..887109 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE55_RS05990 (887134) 887134..887844 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE55_RS05995 (887850) 887850..889583 + 1734 WP_000813208.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T44110 WP_000244777.1 NZ_AP027445:885133-885540 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T44110 NZ_AP027445:885133-885540 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT44110 WP_000354046.1 NZ_AP027445:884886-885152 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT44110 NZ_AP027445:884886-885152 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References