Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 49694..50529 | Replicon | chromosome |
| Accession | NZ_AP027445 | ||
| Organism | Escherichia coli strain EC291 isolate EC291 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A7W4PV54 |
| Locus tag | QUE55_RS01835 | Protein ID | WP_000854821.1 |
| Coordinates | 49694..50071 (-) | Length | 126 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | F4TIK7 |
| Locus tag | QUE55_RS01840 | Protein ID | WP_001285605.1 |
| Coordinates | 50161..50529 (-) | Length | 123 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| QUE55_RS01805 (45335) | 45335..46258 | - | 924 | WP_000535961.1 | carboxylate/amino acid/amine transporter | - |
| QUE55_RS01810 (46369) | 46369..47553 | - | 1185 | WP_001172876.1 | sugar efflux transporter | - |
| QUE55_RS01815 (47950) | 47950..48111 | - | 162 | Protein_47 | RhuM family protein | - |
| QUE55_RS01820 (48348) | 48348..49190 | - | 843 | WP_149506334.1 | DUF4942 domain-containing protein | - |
| QUE55_RS01825 (49275) | 49275..49472 | - | 198 | WP_086795284.1 | DUF957 domain-containing protein | - |
| QUE55_RS01830 (49548) | 49548..49697 | - | 150 | Protein_50 | DUF5983 family protein | - |
| QUE55_RS01835 (49694) | 49694..50071 | - | 378 | WP_000854821.1 | type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA | Toxin |
| QUE55_RS01840 (50161) | 50161..50529 | - | 369 | WP_001285605.1 | type IV toxin-antitoxin system YeeU family antitoxin | Antitoxin |
| QUE55_RS01845 (50609) | 50609..50830 | - | 222 | Protein_53 | DUF987 domain-containing protein | - |
| QUE55_RS01850 (50917) | 50917..51393 | - | 477 | WP_149506335.1 | RadC family protein | - |
| QUE55_RS01855 (51409) | 51409..51888 | - | 480 | WP_001696591.1 | antirestriction protein | - |
| QUE55_RS01860 (51988) | 51988..52128 | + | 141 | WP_000997937.1 | hypothetical protein | - |
| QUE55_RS01865 (52154) | 52154..52972 | - | 819 | WP_001175153.1 | DUF932 domain-containing protein | - |
| QUE55_RS01870 (53062) | 53062..53295 | - | 234 | WP_001278287.1 | DUF905 family protein | - |
| QUE55_RS01875 (53301) | 53301..53978 | - | 678 | WP_001097301.1 | hypothetical protein | - |
| QUE55_RS01880 (54126) | 54126..54806 | - | 681 | WP_001282919.1 | WYL domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 126 a.a. Molecular weight: 13930.95 Da Isoelectric Point: 8.5163
>T44107 WP_000854821.1 NZ_AP027445:c50071-49694 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ
Download Length: 378 bp
>T44107 NZ_AP027445:c50071-49694 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA
Antitoxin
Download Length: 123 a.a. Molecular weight: 13652.48 Da Isoelectric Point: 6.0601
>AT44107 WP_001285605.1 NZ_AP027445:c50529-50161 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRACIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRACIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK
Download Length: 369 bp
>AT44107 NZ_AP027445:c50529-50161 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCTGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCTGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A7W4PV54 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2U2V3N6 |